M-CSF/CSF1 antibody (AA 33-262)

Catalog No. ABIN3042763

This Rabbit Polyclonal antibody specifically detects M-CSF/CSF1 in ELISA and IHC. It exhibits reactivity toward Mouse and Ratand has been mentioned in 1 publication.

Quick Overview for M-CSF/CSF1 antibody (AA 33-262) (ABIN3042763)

Target: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This M-CSF/CSF1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Product Details anti-M-CSF/CSF1 Antibody

Binding Specificity: AA 33-262

Purpose: Anti-MCSF/CSF1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-MCSF/CSF1 Antibody Picoband® (ABIN3042763). Tested in ELISA, IHC applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse MCSF recombinant protein (Position: K33-E262). Mouse MCSF shares 80.9 % and 88.6% amino acid (aa) sequence identity with human and rat MCSF, respectively.

Isotype: IgG

Application Details

Application Notes: ELISA, 0.1-0.5 μg/mL, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
1. Fixe P, Praloran V (June 1997)."Macrophage colony-stimulating-factor (M-CSF or CSF-1) and its receptor: structure-function relationships". Eur. Cytokine Netw. 8 (2): 125-36. 2. Nemunaitis J (April 1993). "Macrophage function activating cytokines: potential clinical application". Crit. Rev. Oncol. Hematol. 14 (2): 153-71. 3. Stanley ER, Berg KL, Einstein DB, Lee PS, Pixley FJ, Wang Y, Yeung YG (January 1997). "Biology and action of colony--stimulating factor-1". Mol. Reprod. Dev. 46(1): 4-10.

Comment:

Antibody can be supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-M-CSF/CSF1 antibody (ABIN3042763)

Pei, Sun, Zhang, Wang, Ren: "Interstitial tumor-associated macrophages combined with tumor-derived colony-stimulating factor-1 and interleukin-6, a novel prognostic biomarker in non-small cell lung cancer." in: The Journal of thoracic and cardiovascular surgery, Vol. 148, Issue 4, pp. 1208-1216.e2, (2015) (PubMed).

Target Details for M-CSF/CSF1

Target: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Alternative Name: Csf1

Background:

Synonyms: Macrophage colony-stimulating factor 1,CSF-1,MCSF,Processed macrophage colony-stimulating factor 1,Csf1,Csfm,

Tissue Specificity: Non-neural epithelial tissues.

Background: M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival ofmonocytes, macrophages, and bone marrow progenitor cells. M-CSF affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor (CSF1R or M-CSF-R encoded by the c-fms proto-oncogene), M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy.

Sequence Similarities: Contains 5 cadherin domains.

Molecular Weight: 130 kDa

Gene ID: 12977

UniProt: P07141

Pathways: RTK Signaling