IL-10 antibody (AA 19-178)

Catalog No. ABIN3042804

This anti-IL-10 antibody is a Rabbit Polyclonal antibody detecting IL-10 in WB, IHC and ELISA. Suitable for Human. This Primary Antibody has been cited in 11+ publications.

Quick Overview for IL-10 antibody (AA 19-178) (ABIN3042804)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-10 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-IL-10 Antibody

Binding Specificity: AA 19-178

Purpose: Anti-Interleukin-10 IL10 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Interleukin-10 IL10 Antibody (ABIN3042804). Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human IL-10 recombinant protein (Position: S19-N178).

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
Western blot, 0.1-0.5 μg/mL, Human
1. Eskdale J, Kube D, Tesch H, Gallagher G (1997). "Mapping of the human IL10 gene and further characterization of the 5' flanking sequence".

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide. Carrier free (No BSA)

Preservative: Sodium azide, Without preservative

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-IL-10 antibody (ABIN3042804)

He, Liang, Guo, Huang, Niu, Dong, Wang, Zhang: "Re-polarizing Myeloid-derived Suppressor Cells (MDSCs) with Cationic Polymers for Cancer Immunotherapy." in: Scientific reports, Vol. 6, pp. 24506, (2017) (PubMed).

Li, Wu, Hang, Zhang, Li: "Women with recurrent spontaneous abortion have decreased 25(OH) vitamin D and VDR at the fetal-maternal interface." in: Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, Vol. 50, Issue 11, pp. e6527, (2017) (PubMed).

Dong, Yin, Qi, Xu, Peng: "Protective Effect of the Total Saponins from Rosa laevigata Michx Fruit against Carbon Tetrachloride-Induced Liver Fibrosis in Rats." in: Nutrients, Vol. 7, Issue 6, pp. 4829-50, (2016) (PubMed).

Li, Tan, Tong, Han, Zhang, Liu, Sun: "Pentoxifylline inhibits pulmonary inflammation induced by infrarenal aorticcross-clamping dependent of adenosine receptor A2A." in: American journal of translational research, Vol. 8, Issue 5, pp. 2210-21, (2016) (PubMed).

Presti, DOrazio, Labra, La Ferla, Mezzasalma, Bizzaro, Giardina, Michelotti, Tursi, Vassallo, Di Gennaro: "Evaluation of the probiotic properties of new Lactobacillus and Bifidobacterium strains and their in vitro effect." in: Applied microbiology and biotechnology, Vol. 99, Issue 13, pp. 5613-26, (2015) (PubMed).

Gong, Wang, Yuan, Li, Gu, Zhao, Zhang, Jia, Feng, Liu: "Inhibition of Tumor Growth and Immunomodulatory Effects of Flavonoids and Scutebarbatines of Scutellaria barbata D. Don in Lewis-Bearing C57BL/6 Mice." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 630760, (2015) (PubMed).

Wang, Deng, Ren, Xiao, Liu, Tao: "Exogenous interleukin-10 alleviates allergic inflammation but inhibits local interleukin-10 expression in a mouse allergic rhinitis model." in: BMC immunology, Vol. 15, pp. 9, (2014) (PubMed).

Wang, Zhou, Chen, Zhu: "Putative role of ischemic postconditioning in a rat model of limb ischemia and reperfusion: involvement of hypoxia-inducible factor-1? expression." in: Brazilian journal of medical and biological research = Revista brasileira de pesquisas me?dicas e biolo?gicas / Sociedade Brasileira de Biofi?sica ... [et al.], Vol. 47, Issue 9, pp. 738-45, (2014) (PubMed).

Jin, Yang, Song, Zhang, Li: "Protective roles of quercetin in acute myocardial ischemia and reperfusion injury in rats." in: Molecular biology reports, Vol. 39, Issue 12, pp. 11005-9, (2012) (PubMed).

Wang, Lu, Zhang, Chen, Luo, Qin, Chen: "Increased IL-10 mRNA expression in tumor-associated macrophage correlated with late stage of lung cancer." in: Journal of experimental & clinical cancer research : CR, Vol. 30, pp. 62, (2011) (PubMed).

Zhang, Leng, Xue, Zhang, Wang, Kang: "Effects of penehyclidine hydrochloride in small intestinal damage caused by limb ischemia-reperfusion." in: World journal of gastroenterology, Vol. 17, Issue 2, pp. 254-9, (2011) (PubMed).

Target Details for IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: IL10

Background:

Synonyms: Interleukin-10,IL-10,Cytokine synthesis inhibitory factor,CSIF,IL10,

Tissue Specificity: Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.

Background: Interleukin-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-gamma, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and regulatory T-cells.IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al. (1992) showed that the mouse IL 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al. (1997) mapped the IL10 gene to the junction between 1q31 and 1q32.

Sequence Similarities: Belongs to the IL-10 family.

Molecular Weight: 19 kDa

UniProt: P22301

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints