PROX1 antibody (Middle Region)

Catalog No. ABIN3042819

This anti-PROX1 antibody is a Rabbit Polyclonal antibody detecting PROX1 in WB. Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 1 publication.

Quick Overview for PROX1 antibody (Middle Region) (ABIN3042819)

Target: PROX1 (Prospero Homeobox 1 (PROX1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PROX1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PROX1 Antibody

Binding Specificity: AA 346-359, Middle Region

Purpose: Anti-Prospero homeobox protein 1 PROX1 Antibody Picoband®

Sequence: KHLAETLKQE LNTA

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Prospero homeobox protein 1 PROX1 Antibody (ABIN3042819). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PROX1, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Dyer, M. A., Livesey, F. J., Cepko, C. L., Oliver, G. Prox1 function controls progenitor cell proliferation and horizontal cell genesis in the mammalian retina. Nature Genet. 34: 53-58, 2003. 2. Harvey, N. L., Srinivasan, R. S., Dillard, M. E., Johnson, N. C., Witte, M. H., Boyd, K., Sleeman, M. W., Oliver, G. Lymphatic vascular defects promoted by Prox1 haploinsufficiency cause adult-onset obesity. Nature Genet. 37: 1072-1081, 2005. 3. Kazenwadel, J., Michael, M. Z., Harvey, N. L. Prox1 expression is negatively regulated by miR-181 in endothelial cells. Blood 116: 2395-2401, 2010.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-PROX1 antibody (ABIN3042819)

Yang, Gao, Ma, Teng, Liu, Wei, Lu, Cheng, Hou, Zou: "Fucoidan inhibits lymphangiogenesis by downregulating the expression of VEGFR3 and PROX1 in human lymphatic endothelial cells." in: Oncotarget, Vol. 7, Issue 25, pp. 38025-38035, (2018) (PubMed).

Target Details for PROX1

Target: PROX1 (Prospero Homeobox 1 (PROX1))

Alternative Name: PROX1

Background:

Synonyms: Prospero homeobox protein 1,Homeobox prospero-like protein PROX1,PROX-1,PROX1,

Tissue Specificity: Most actively expressed in the developing lens. Detected also in embryonic brain, lung, liver and kidney. In adult, it is more abundant in heart and liver than in brain, skeletal muscle, kidney and pancreas. .

Background: Prospero homeobox protein 1, also called PROX1 is a protein that in humans is encoded by the PROX1 gene. This gene is mapped to 1q32.3. The protein encoded by this gene is a member of the homeobox transcription factor family. Members of this family contain a homeobox domain that consists of a 60-amino acid helix-turn-helix structure that binds DNA and RNA. The protein encoded by this gene is conserved across vertebrates and may play an essential role during development. Altered levels of this protein have been reported in cancers of different organs, such as colon, brain, blood, breast, pancreas, liver and esophagus.

Sequence Similarities: Belongs to the Prospero homeobox family.

Molecular Weight: 55 kDa

UniProt: Q92786

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation