UBD antibody (N-Term)

Catalog No. ABIN3042826

This Rabbit Polyclonal antibody specifically detects UBD in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for UBD antibody (N-Term) (ABIN3042826)

Target: UBD (Ubiquitin D (UBD))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-UBD Antibody

Binding Specificity: N-Term

Purpose: Anti-Diubiquitin/UBD Antibody

Sequence: YDSVKKIKEH VRSK

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related mouse,rat sequences by five amino acids.

Characteristics: Anti-Diubiquitin/UBD Antibody. Tested in IHC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Diubiquitin, different from the related mouse and rat sequences by five amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, HumanWestern blot, 0.1-0.5 μg/mL, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for UBD

Target: UBD (Ubiquitin D (UBD))

Alternative Name: UBD

Background:

Background: Ubiquitin D, also called UBD or FAT10 is a protein that in humans is encoded by the UBD gene. This gene is mapped to 6p22.1. Ubiquitin-like protein modifier can be covalently attached to target protein and subsequently leads to their degradation by the 26S proteasome in a NUB1L-dependent manner. This gene may be involved in dendritic cell (DC) maturation, the process by which immature dendritic cells differentiate into fully competent antigen-presenting cells that initiate T-cell responses. It may be involved in the formation of aggresomes when proteasome is saturated or impaired. This gene mediates apoptosis in a caspase-dependent manner, especially in renal epithelium and tubular cells during renal diseases such as polycystic kidney disease and Human immunodeficiency virus (HIV)-associated nephropathy (HIVAN). It probably functions as a survival factor.

Gene Full Name: ubiquitin like modifier D

Sequence Similarities: Contains 2 ubiquitin-like domains.

Molecular Weight: 91 kDa

Gene ID: 10537

UniProt: O15205

Pathways: Ubiquitin Proteasome Pathway