KLF4 antibody (N-Term)

Catalog No. ABIN3042832

Product Details anti-KLF4 Antibody

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Binding Specificity: AA 93-108, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLF4 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Krueppel-like factor 4(KLF4) detection. Tested with WB in Human,Mouse,Rat.

Sequence: RRETEEFNDL LDLDFI

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse, rat
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Krueppel-like factor 4(KLF4) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: Kruppel-like factor 4(gut)
Protein Name: Krueppel-like factor 4

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human KLF4(93-108aa RRETEEFNDLLDLDFI), identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-KLF4 antibody (ABIN3042832)

Ai, Shao, Shi, Yu, Wu, Du, Zhang, Guo: "Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901." in: Stem cells international, Vol. 2016, pp. 1792573, (2016) (PubMed).

Ai, Shao, Wu, Yu, Du, Shi, Shi, Zhang, Guo: "CHIR99021 enhances Klf4 Expression through β-Catenin Signaling and miR-7a Regulation in J1 Mouse Embryonic Stem Cells." in: PLoS ONE, Vol. 11, Issue 3, pp. e0150936, (2016) (PubMed).

Target Details for KLF4

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Alternative Name: KLF4 (KLF4 Products)

Synonyms: EZF antibody, GKLF antibody, Gklf antibody, Zie antibody, KLF4 antibody, biklf antibody, biklf-A antibody, Kruppel like factor 4 antibody, Kruppel-like factor 4 (gut) antibody, Kruppel-like factor 4 antibody, Kruppel-like factor 4 (gut) L homeolog antibody, KLF4 antibody, Klf4 antibody, klf4 antibody, klf4.L antibody

Background: Kruppel-like factor 4, also known as EZF or GKLF, is a protein that in humans is encoded by the KLF4 gene. This gene is mapped to 9q31.2. KLF4 gene encodes a member of the Kruppel family of transcription factors. This gene plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. It is required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. This gene involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.

Synonyms: Endothelial Kruppel like zinc finger protein antibody|Epithelial zinc finger protein EZF antibody|EZF antibody|GKLF antibody|Gut-enriched krueppel-like factor antibody|KLF antibody|KLF4 antibody|KLF4_HUMAN antibody|Krueppel-like factor 4 antibody|Kruppel like factor 4(Epithelial zinc finger protein EZF)(Gut enriched Krueppel like factor) antibody|Kruppel like factor 4(gut) antibody

UniProt: O43474

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance