Leupaxin antibody (N-Term)

Catalog No. ABIN3042842

The Rabbit Polyclonal anti-Leupaxin antibody has been validated for WB and FACS. It is suitable to detect Leupaxin in samples from Human and Mouse.

Quick Overview for Leupaxin antibody (N-Term) (ABIN3042842)

Target: Leupaxin (LPXN)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Leupaxin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-Leupaxin Antibody

Binding Specificity: AA 115-129, N-Term

Purpose: Anti-Leupaxin/LPXN Antibody Picoband®

Sequence: KKHLPDKQDH KASLD

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Leupaxin/LPXN Antibody (ABIN3042842). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Leupaxin, different from the related rat sequence by two amino acids, and from the related mouse sequence by three amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
1. Lipsky, B. P., Beals, C. R., Staunton, D. E. Leupaxin is a novel LIM domain protein that forms a complex with PYK2. J. Biol. Chem. 273: 11709-11713, 1998. 2. "Entrez Gene: LPXN leupaxin".

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Leupaxin

Target: Leupaxin (LPXN)

Alternative Name: LPXN

Background:

Synonyms: Leupaxin,LPXN,LDLP,

Tissue Specificity: Macrophages, monocytes and osteoclasts (at protein level). Strongly expressed in cells and tissues of hematopoietic origin. Highest expression in lymphoid tissues such as spleen, lymph node, thymus and appendix and in the vascular smooth muscle. Lower levels in bone marrow and fetal liver. Also expressed in peripheral blood lymphocytes and a number of hematopoietic cell lines. Very low levels found in epithelial cell lines. Expressed in prostate cancer (PCa) cells and its expression intensity is ly linked to PCa progression. .

Background: Leupaxin is a protein that in humans is encoded by the LPXN gene. This gene is mapped to 11q12.1. The product encoded by this gene is preferentially expressed in hematopoietic cells and belongs to the paxillin protein family. Similar to other members of this focal-adhesion-associated adaptor-protein family, it has four leucine-rich LD-motifs in the N-terminus and four LIM domains in the C-terminus. It may function in cell type-specific signaling by associating with PYK2, a member of focal adhesion kinase family. As a substrate for a tyrosine kinase in lymphoid cells, this protein may also function in, and be regulated by, tyrosine kinase activity.

Sequence Similarities: Belongs to the paxillin family.

Molecular Weight: 43 kDa

UniProt: O60711