PFN2 antibody (C-Term)

Catalog No. ABIN3042861

The Rabbit Polyclonal anti-PFN2 antibody (ABIN3042861) specifically detects PFN2 in WB, IHC and IF. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for PFN2 antibody (C-Term) (ABIN3042861)

Target: PFN2 (Profilin 2 (PFN2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFN2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PFN2 Antibody

Binding Specificity: C-Term

Purpose: Anti-Profilin 2/PFN2 Antibody

Sequence: NKKAYSMAKY LRDSGF

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related mouse,rat sequences.

Characteristics: Anti-Profilin 2/PFN2 Antibody. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Profilin 2, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunofluorescence, 5 μg/mL, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PFN2

Target: PFN2 (Profilin 2 (PFN2))

Alternative Name: PFN2

Background:

Background: Profilin-2, also called PFN2, is a protein that in humans is encoded by the PFN2 gene. The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. This gene is mapped to 3q25.1. It is though to regulate actin polymerization in response to extracellular signals. There are two alternatively spliced transcript variants encoding different isoforms described for this gene. It binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.

Gene Full Name: profilin 2

Sequence Similarities: Contains 17 laminin EGF-like domains.

Molecular Weight: 15 kDa

Gene ID: 5217

UniProt: P35080

Pathways: Regulation of Actin Filament Polymerization, Synaptic Vesicle Exocytosis