Aquaporin 10 antibody (C-Term)

Catalog No. ABIN3042903

This anti-Aquaporin 10 antibody is a Rabbit Polyclonal antibody detecting Aquaporin 10 in WB. Suitable for Human.

Quick Overview for Aquaporin 10 antibody (C-Term) (ABIN3042903)

Target: Aquaporin 10 (AQP10)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aquaporin 10 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Aquaporin 10 Antibody

Binding Specificity: AA 286-301, C-Term

Purpose: Anti-Aquaporin 10/AQP10 Antibody Picoband®

Sequence: SELETPASAQ MLECKL

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Aquaporin 10/AQP10 Antibody (ABIN3042903). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 10.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Hatakeyama, S., Yoshida, Y., Tani, T., Koyama, Y., Nihei, K., Ohshiro, K., Kamiie, J.-I., Yaoita, E., Suda, T., Hatakeyama, K., Yamamoto, T. Cloning of a new aquaporin (AQP10) abundantly expressed in duodenum and jejunum. Biochem. Biophys. Res. Commun. 287: 814-819, 2001.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Aquaporin 10

Target: Aquaporin 10 (AQP10)

Alternative Name: AQP10

Background:

Synonyms: Aquaporin-10,AQP-10,Aquaglyceroporin-10,Small intestine aquaporin,AQP10,

Tissue Specificity: Expressed exclusively in duodenum and jejunum. Highest expression in absorptive epithelial cells at the tips of villi in the jejunum.

Background: AQP10 was identified in human small intestine. This gene encoded a 264-amino-acid protein with high sequence identity with AQP3 (53 % ), 9 (52 %), and 7 (43 %). These AQPs constitute one subfamily of AQP family that is differentiated from the other subfamily of AQP (AQP0, 1, 2, 4, 5, 6, and 8) by sequence homology. Northern blot analysis revealed expression of a 2.3-kb AQP10 transcript in jejunum but not liver. RNase protection analysis detected nearly exclusive expression in duodenum and jejunum. In situ hybridization analysis demonstrated highest expression of AQP10 in absorptive epithelial cells at the tips of villi in the jejunum. SDS-PAGE analysis showed expression of a 28-kD AQP10 protein, similar to its predicted size as well as to that of the other AQPs. Functional analysis indicated that cells expressing AQP10 are permeable to relatively low amounts of water, but, unlike AQP3, AQP7, and AQP9, are not permeable to urea or glycerol.

Molecular Weight: 31 kDa