SPARCL1 antibody (C-Term)

Catalog No. ABIN3042909

The Rabbit Polyclonal anti-SPARCL1 antibody (ABIN3042909) specifically detects SPARCL1 in WB and IHC. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for SPARCL1 antibody (C-Term) (ABIN3042909)

Target: SPARCL1 (SPARC-Like 1 (Hevin) (SPARCL1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPARCL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SPARCL1 Antibody

Binding Specificity: C-Term

Purpose: Anti-SPARC-like protein 1 SPARCL1 Antibody

Sequence: HCFGIKEEDI DENLLF

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related mouse,rat sequences.

Characteristics: Anti-SPARC-like protein 1 SPARCL1 Antibody. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human SPARCL1, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, MouseWestern blot, 0.1-0.5 μg/mL, Human, Mouse, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SPARCL1

Target: SPARCL1 (SPARC-Like 1 (Hevin) (SPARCL1))

Alternative Name: SPARCL1

Background:

Background: SPARCL1 (SPARC-Like Protein 1), also known as HEVIN, is a protein that in humans is encoded by the SPARCL1 gene. The cells in high endothelial venules (HEVs) in lymphoid tissues have a plump, almost cuboidal, appearance and support high levels of lymphocyte extravasation from blood, possibly due to the presence of desmosome-like junctions rather than tight junctions in the HEVs. In chronic inflammation, the activated endothelium of nonlymphoid tissues acquires an HEV-like morphology and function. Hevin is highly expressed in HEV and is though to contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration (Girard and Springer, 1995).

Gene Full Name: SPARC like 1

Sequence Similarities: Belongs to the SPARC family.

Molecular Weight: 95 kDa

Gene ID: 8404

UniProt: Q14515