Prothrombin Fragment 2 (C-Term) antibody

Catalog No. ABIN3042926

There is 1 publication for this product available. The Rabbit Polyclonal anti-Prothrombin Fragment 2 antibody is suitable to detect Prothrombin Fragment 2 in samples from Rat. It has been validated for WB.

Quick Overview for Prothrombin Fragment 2 (C-Term) antibody (ABIN3042926)

Target: Prothrombin Fragment 2

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Binding Specificity: C-Term

Purpose: Anti-Prothrombin/F2 Antibody

Sequence: THVFRLKRWM QKV

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Prothrombin/F2 Antibody. Tested in WB applications. This antibody reacts with Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of rat Prothrombin.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References

Shen, Fang, Lv, Pei, Wang, Jiang, Ding: "Heparin impairs angiogenesis through inhibition of microRNA-10b." in: The Journal of biological chemistry, Vol. 286, Issue 30, pp. 26616-27, (2011) (PubMed).

Target Details

Target: Prothrombin Fragment 2

Background:

Background: F2 (Coagulation Factor II), also known as thrombin, is a serine protease that in humans is encoded by the F2 gene. Royle et al. (1987) assigned the gene for human prothrombin (F2) to chromosome 11p11-q12 by analysis of a panel of somatic cell hybrid DNAs and by in situ hybridization, using both cDNA and genomic probes. The activated thrombin enzyme plays an important role in hemostasis and thrombosis: it converts fibrinogen to fibrin for blood clot formation, stimulates platelet aggregation, and activates coagulation factors V, VIII (F8), and XIII (F13A1). Thrombin also inhibits coagulation by activating protein C.

Gene Full Name: coagulation factor II(thrombin)

Sequence Similarities: Belongs to the peptidase S1 family.

Molecular Weight: 100 kDa

Gene ID: 29251

UniProt: P18292