Kallikrein 1 antibody (AA 25-261)

Catalog No. ABIN3042937

This Rabbit Polyclonal antibody specifically detects Kallikrein 1 in WB and IHC. It exhibits reactivity toward Mouse and has been mentioned in 2+ publications.

Quick Overview for Kallikrein 1 antibody (AA 25-261) (ABIN3042937)

Target: Kallikrein 1 (KLK1)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Kallikrein 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Kallikrein 1 Antibody

Binding Specificity: AA 25-261

Purpose: Anti-Kallikrein 1/KLK1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Kallikrein 1/KLK1 Antibody (ABIN3042937). Tested in IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse KLK1 recombinant protein (Position: I25-D261).

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse
Western blot, 0.1-0.5 μg/mL, Mouse
1. Azizi, M., Boutouyrie, P., Bissery, A., Agharazii, M., Verbeke, F., Stern, N., Bura-Riviere, A., Laurent, S., Alhenc-Gelas, F., Jeunemaitre, X. Arterial and renal consequences of partial genetic deficiency in tissue kallikrein activity in humans. J. Clin. Invest. 115: 780-787, 2005. 2. Baker, A. R., Shine, J. Human kidney kallikrein: cDNA cloning and sequence analysis. DNA 4: 445-450, 1985. 3. Clements J, Hooper J, Dong Y, Harvey T (2001). "The expanded human kallikrein (KLK) gene family: genomic organisation, tissue-specific expression and potential functions".

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-Kallikrein 1 antibody (ABIN3042937)

Zhang, Xu, Zhao, Chen: "Genistein improves inflammatory response and colonic function through NF-κB signal in DSS-induced colonic injury." in: Oncotarget, Vol. 8, Issue 37, pp. 61385-61392, (2017) (PubMed).

Lin, Lin, Chung, Vonderfecht, Camden, Flodby, Borok, Limesand, Mizushima, Ann: "Dynamic involvement of ATG5 in cellular stress responses." in: Cell death & disease, Vol. 5, pp. e1478, (2014) (PubMed).

Target Details for Kallikrein 1

Target: Kallikrein 1 (KLK1)

Alternative Name: KLK1

Background:

Synonyms: Kallikrein-1,3.4.21.35,Glandular kallikrein K1,KAL-B,Renal kallikrein,Tissue kallikrein-6,mGK-6,Klk1,Klk-6, Klk6,

Background: KLK1 (KALLIKREIN 1), also called KLKR, is a protein that in humans is encoded by the KLK1 gene. KLK1 is a member of the peptidase S1 family. KLK1 is a serine protease that generates Lys-bradykinin by specific proteolysis of kininogen-1. The KLK1 gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19 and its exact cytogenetic location is 19q13.33. The KLK1 gene contains 5 coding exons. And KLK1 is the most centromeric gene in the cluster. Mice lacking tissue kallikrein are unable to generate significant levels of kinins in most tissues and develop cardiovascular abnormalities early in adulthood despite normal blood pressure. The protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen.

Sequence Similarities: Belongs to the peptidase S1 family. Kallikrein subfamily.

Molecular Weight: 29 kDa

UniProt: P15947

Pathways: Complement System