T-Bet antibody (C-Term)

Catalog No. ABIN3042957

This anti-T-Bet antibody is a Rabbit Polyclonal antibody detecting T-Bet in WB. Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 1 publication.

Quick Overview for T-Bet antibody (C-Term) (ABIN3042957)

Target: T-Bet

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This T-Bet antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-T-Bet Antibody

Binding Specificity: AA 522-535, C-Term

Purpose: Anti-T-bet/Tbx21 Antibody Picoband®

Sequence: DKEAEGQFYN YFPN

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-T-bet/Tbx21 Antibody (ABIN3042957). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human T-bet, different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Finotto, S., Neurath, M. F., Glickman, J. N., Qin, S., Lehr, H. A., Green, F. H. Y., Ackerman, K., Haley, K., Galle, P. R., Szabo, S. J., Drazen, J. M., De Sanctis, G. T., Glimcher, L. H. Development of spontaneous airway changes consistent with human asthma in mice lacking T-bet. Science 295: 336-338, 2002. 2. Szabo, S. J., Kim, S. T., Costa, G. L., Zhang, X., Fathman, C. G., Glimcher, L. H. A novel transcription factor, T-bet, s Th1 lineage commitment. Cell 100: 655-669, 2000. 3. Zhang, W., Yang, S. Y. Cloning and characterization of a new member of the T-box gene family. Genomics 70: 41-48, 2000.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-T-Bet antibody (ABIN3042957)

Miao, Run-Ming, Yi: "T-Bet Mediated Anti-Neoplastic Effects of Dendritic Cell-Cytokine Induced Killer Cells in vitro." in: Iranian journal of pediatrics, Vol. 22, Issue 1, pp. 43-51, (2012) (PubMed).

Target Details for T-Bet

Target: T-Bet

Alternative Name: TBX21

Background:

Synonyms: T-box transcription factor TBX21,T-box protein 21,T-cell-specific T-box transcription factor T-bet,Transcription factor TBLYM,TBX21,TBET, TBLYM,

Tissue Specificity: T-cell specific.

Background: TBX21 (T-Box 21), also called TBET, is a protein that in humans is encoded by the TBX21 gene. This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. Zhang and Yang (2000) mapped the mouse Tbx21 gene to chromosome 11D in an area showing homology of synteny with human chromosome 17. Szabo et al. (2000) showed that TBX21 expression correlates with IFNG expression in Th1 and natural killer (NK) cells. Ectopic expression of TBX21 both transactivated the IFNG gene and induced endogenous IFNG production. Finotto et al. (2002) observed reduced expression of the T (H)1 transcription factor Tbet in T cells from airways of patients with asthma compared with that in T cells from airways of nonasthmatic patients, suggesting that loss of Tbet might be associated with asthma.

Sequence Similarities: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.

Molecular Weight: 58-60 kDa