TJP3 antibody (C-Term)

Catalog No. ABIN3042974

The Rabbit Polyclonal anti-TJP3 antibody is suitable to detect TJP3 in samples from Human. It has been validated for WB, IHC, ICC, FACS and IF.

Quick Overview for TJP3 antibody (C-Term) (ABIN3042974)

Target: TJP3 (Tight Junction Protein 3 (Zona Occludens 3) (TJP3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TJP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-TJP3 Antibody

Binding Specificity: C-Term

Purpose: Anti-Zonula occludens protein 3/TJP3 Antibody

Sequence: RVHDAESSDE DGYDWGPATD L

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Zonula occludens protein 3/TJP3 Antibody. Tested in Flow Cytometry, ICC/IF, IHC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Zonula occludens protein 3.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for TJP3

Target: TJP3 (Tight Junction Protein 3 (Zona Occludens 3) (TJP3))

Alternative Name: TJP3

Background:

Background: TJP3 (TIGHT JUNCTION PROTEIN 3), also called ZO3, is a protein that in humans is encoded by the TJP3 gene. TJP3 is a member of the family of membrane-associated guanylate kinase-like proteins (MAGUK) that associate with intracellular junctions (Itoh et al., 1999). Haskins et al. (1998) showed that recombinant Tjp3 binds in vitro translated Tjp1 and binds ly to the cytoplasmic tail of occludin but no binding was detected between Tjp3 and Tjp2. Roh et al. (2002) determined that Tjp3 interacted with PATJ (INADL) and that both proteins colocalized to tight junctions in vivo. In vitro studies showed that the sixth and eighth PDZ domains of PATJ mediated interaction with the Tjp3 C-terminal domain and with Cldn1, respectively.

Gene Full Name: tight junction protein 3

Sequence Similarities: Belongs to the MAGUK family.

Molecular Weight: 130-140 kDa

Gene ID: 27134

UniProt: O95049