OSBP antibody (Middle Region)

Catalog No. ABIN3042983

Product Details anti-OSBP Antibody

Target: OSBP (Oxysterol Binding Protein (OSBP))

Binding Specificity: AA 290-304, Middle Region

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OSBP antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Oxysterol-binding protein 1(OSBP) detection. Tested with WB in Human,Mouse,Rat.

Sequence: WQKSLQYERD QRIRL

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Oxysterol-binding protein 1(OSBP) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: oxysterol binding protein
Protein Name: Oxysterol-binding protein 1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human OSBP1(290-304aa WQKSLQYERDQRIRL), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for OSBP

Target: OSBP (Oxysterol Binding Protein (OSBP))

Alternative Name: OSBP (OSBP Products)

Synonyms: CG6708 antibody, Dmel\\CG6708 antibody, OSBP-Dm antibody, osbp antibody, OSBP1 antibody, 1110018F06Rik antibody, AW559088 antibody, mKIAA4220 antibody, Oxysterol binding protein antibody, oxysterol binding protein antibody, Osbp antibody, OSBP antibody

Background: OSBP(Oxysterol-Binding Protein), also called OSBP1, is a protein that in humans is encoded by the OSBP gene. Im et al.(2005) reported the structure of the full-length yeast oxysterol-binding-protein Osh4, a member of the OSBP-related protein(ORP) family, at 1.5- to 1.9-angstrom resolution in complexes with ergosterol, cholesterol, and 7-, 20- and 25-hydroxy cholesterol. Moreira et al.(2001) refined the localization of the OSBP1 gene to chromosome 11q12.1 by radiation hybrid analysis. Wang et al.(2005) found that OSBP functions as a cholesterol-binding scaffolding protein coordinating the activity of 2 phosphatases to control the extracellular signal-regulated kinase(ERK) signaling pathway. Cytosolic OSBP formed an approximately 440-kD oligomer with a member of the PTPPBS family of tyrosine phosphatases, the serine/threonine phosphatase PP2A, and cholesterol.

Synonyms: OSBP 1 antibody|OSBP antibody|OSBP1_HUMAN antibody|Oxysterol binding protein 1 antibody|Oxysterol-binding protein 1 antibody

UniProt: P22059