FMO4 antibody (N-Term)

Catalog No. ABIN3043006

Product Details anti-FMO4 Antibody

Target: FMO4 (Flavin Containing Monooxygenase 4 (FMO4))

Binding Specificity: AA 75-92, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FMO4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Anti-Flavin containing monooxygenase 4/FMO4 Antibody Picoband®

Sequence: HEDYPNFMNH EKFWDYLQ

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Flavin containing monooxygenase 4/FMO4 Antibody (ABIN3043006). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Flavin containing monooxygenase 4, different from the related rat sequence by two amino acids, and from the related mouse sequence by three amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Rat, Human
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Dolphin, C. T., Shephard, E. A., Povey, S., Smith, R. L., Phillips, I. R. Cloning, primary sequence and chromosomal localization of human FMO2, a new member of the flavin-containing mono-oxygenase family. Biochem. J. 287: 261-267, 1992. 2. Lawton, M. P., Cashman, J. R., Cresteil, T., Dolphin, C. T., Elfarra, A. A., Hines, R. N., Hodgson, E., Kimura, T., Ozols, J., Phillips, I. R., Philpot, R. M., Poulsen, L. L., Rettie, A. E., Shephard, E. A., Williams, D. E., Ziegler, D. M. A nomenclature for the mammalian flavin-containing monooxygenase gene family based on amino acid sequence identities. Arch. Biochem. Biophys. 308: 254-257, 1994. 3. Phillips, I. R., Dolphin, C. T., Clair, P., Hadley, M. R., Hutt, A. J., McCombie, R. R., Smith, R. L., Shephard, E. A.The molecular biology of the flavin-containing monooxygenases of man. Chem. Biol. Interact. 96: 17-32, 1995.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for FMO4

Target: FMO4 (Flavin Containing Monooxygenase 4 (FMO4))

Alternative Name: FMO4 (FMO4 Products)

Background:

Synonyms: Dimethylaniline monooxygenase [N-oxide-forming] 4,1.14.13.8,Dimethylaniline oxidase 4,Hepatic flavin-containing monooxygenase 4,FMO 4,FMO4,FMO2,

Tissue Specificity: Liver.

Background: FMO4 (Flavin-containing monooxygenase 4) also known as FMO2, FORMERLY or FMO, ADULT LIVER FORM, is an enzyme that in humans is encoded by the FMO4 gene. By PCR analysis, Dolphin et al. (1992) mapped the FMO4 gene to chromosome 1. Southern blot hybridization with single exon probes demonstrated that human FMO4 and FMO1 are, in each case, the product of a single gene. Lawton et al. (1994) proposed a reclassification of mammalian FMOs. Under this system, FMO2 described by Dolphin et al. (1992) is now designated FMO4.

Sequence Similarities: Belongs to the FMO family.

Molecular Weight: 95 kDa

UniProt: P31512