CXCR2 antibody (Middle Region)

Catalog No. ABIN3043018

This Rabbit Polyclonal antibody specifically detects CXCR2 in WB. It exhibits reactivity toward Human and Rat. It has been mentioned in 5+ publications

Quick Overview for CXCR2 antibody (Middle Region) (ABIN3043018)

Target: CXCR2 (Chemokine (C-X-C Motif) Receptor 2 (CXCR2))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCR2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CXCR2 Antibody

Binding Specificity: Middle Region

Purpose: Anti-CXCR2 Antibody

Sequence: CYEDMGNNTA NWRMLLR

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related mouse sequence by six amino acids.

Characteristics: Anti-CXCR2 Antibody. Tested in WB applications. This antibody reacts with Human, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human CXCR2, different from the related mouse sequence by six amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-CXCR2 antibody (ABIN3043018)

Zhou, Li, Li, Liu, Zhu, Ju, Miao, Xu: "Overexpression of GRK6 attenuates neuropathic pain via suppression of CXCR2 in rat dorsal root ganglion." in: Molecular pain, Vol. 12, (2016) (PubMed).

Xu, Zhu, Zhang, Tian, Zhang, Wu, Gao: "NF?B-mediated CXCL1 production in spinal cord astrocytes contributes to the maintenance of bone cancer pain in mice." in: Journal of neuroinflammation, Vol. 11, pp. 38, (2014) (PubMed).

Cao, Zhang, Xie, Jiang, Ji, Gao: "Chemokine CXCL1 enhances inflammatory pain and increases NMDA receptor activity and COX-2 expression in spinal cord neurons via activation of CXCR2." in: Experimental neurology, Vol. 261, pp. 328-36, (2014) (PubMed).

Chen, Park, Xie, Berta, Nedergaard, Ji: "Connexin-43 induces chemokine release from spinal cord astrocytes to maintain late-phase neuropathic pain in mice." in: Brain : a journal of neurology, Vol. 137, Issue Pt 8, pp. 2193-209, (2014) (PubMed).

Zhang, Cao, Zhang, Ji, Gao: "Chemokine contribution to neuropathic pain: respective induction of CXCL1 and CXCR2 in spinal cord astrocytes and neurons." in: Pain, Vol. 154, Issue 10, pp. 2185-97, (2013) (PubMed).

Target Details for CXCR2

Target: CXCR2 (Chemokine (C-X-C Motif) Receptor 2 (CXCR2))

Alternative Name: CXCR2

Background:

Background: CXCR2 is a receptor for Interleukin 8, which is a powerful neutrophil chemotactic factor. It is a member of the GPCR family (subfamily, chemokine). Binding of IL8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL8 with a high affinity and to GRO/MGSA and NAP2 also with a high affinity. It has been reported to be expressed in a wide variety of tissues. ESTs have been isolated from human placenta and thymus libraries.

Gene Full Name: C-X-C motif chemokine receptor 2

Sequence Similarities: Belongs to the G-protein coupled receptor 1 family.

Molecular Weight: 45 kDa

Gene ID: 3579

UniProt: P25025

Pathways: cAMP Metabolic Process