Neurotrophin 3 antibody (Middle Region)

Catalog No. ABIN3043024

This Rabbit Polyclonal antibody specifically detects Neurotrophin 3 in WB and IHC. It exhibits reactivity toward Human, Mouse and Ratand has been mentioned in 1 publication.

Quick Overview for Neurotrophin 3 antibody (Middle Region) (ABIN3043024)

Target: Neurotrophin 3 (NTF3)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Neurotrophin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Neurotrophin 3 Antibody

Binding Specificity: AA 174-189, Middle Region

Purpose: Anti-Neurotrophin 3/NTF3 Antibody Picoband®

Sequence: TVLGEIKTGN SPVKQY

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Neurotrophin 3/NTF3 Antibody (ABIN3043024). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Neurotrophin 3, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Rat, Human, Mouse
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Jones, K. R., Reichardt, L. F. : Molecular cloning of a human gene that is a member of the nerve growth factor family. Proc. Nat. Acad. Sci. 87: 8060-8064, 1990. 2. Rosenthal A., Goeddel D.V., Nguyen T., Lewis M., Shih A., Laramee G.R., Nikolics K., Winslow J.W.,"Primary structure and biological activity of a novel human neurotrophic factor.",Neuron 4:767-773(1990).

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Neurotrophin 3 antibody (ABIN3043024)

Guo, Zeng, Li, Huang, Liu, Li, Ding, Wu, Cai: "Cotransplant of neural stem cells and NT-3 gene modified Schwann cells promote the recovery of transected spinal cord injury." in: Spinal cord, Vol. 45, Issue 1, pp. 15-24, (2007) (PubMed).

Target Details for Neurotrophin 3

Target: Neurotrophin 3 (NTF3)

Alternative Name: NTF3

Background:

Synonyms: Neurotrophin-3,NT-3,HDNF,Nerve growth factor 2,NGF-2,Neurotrophic factor,NTF3,

Tissue Specificity: Brain and peripheral tissues.

Background: Neurotrophin-3 is a member of a family of neurotrophic factors, that is closely related to both nerve growth factor and brain derived neurotrophic factor. These proteins are involved in the maintenance of the adult nervous system and affect development of neurons in the embryo when it is expressed in human placenta. NT3 deficient mice generated by gene targeting display severe movement defects of the limbs.

Sequence Similarities: Belongs to the enolase family.

Molecular Weight: 47 kDa

UniProt: P20783

Pathways: RTK Signaling, Neurotrophin Signaling Pathway