NOTCH4 antibody (C-Term)

Catalog No. ABIN3043028

The Rabbit Polyclonal anti-NOTCH4 antibody has been validated for WB. It is suitable to detect NOTCH4 in samples from Human.

Quick Overview for NOTCH4 antibody (C-Term) (ABIN3043028)

Target: NOTCH4 (Notch 4 (NOTCH4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOTCH4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NOTCH4 Antibody

Binding Specificity: AA 1805-1818, C-Term

Purpose: Anti-NOTCH4 Antibody Picoband®

Sequence: DVAHQRNHWD LLTL

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-NOTCH4 Antibody (ABIN3043028). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human NOTCH4.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Li, L., Huang, G. M., Banta, A. B., Deng, Y., Smith, T., Dong, P., Friedman, C., Chen, L., Trask, B. J., Spies, T., Rowen, L., Hood, L. Cloning, characterization, and the complete 56.8-kilobase DNA sequence of the human NOTCH4 gene. Genomics 51: 45-58, 1998. 2. Murphy, P. A., Lam, M. T. Y., Wu, X., Kim, T. N., Vartanian, S. M., Bollen, A. W., Carlson, T. R., Wang, R. A. Endothelial Notch4 signaling induces hallmarks of brain arteriovenous malformations in mice. Proc. Nat. Acad. Sci. 105: 10901-10906, 2008. 3. Wei, J., Hemmings, G. P. The NOTCH4 locus is associated with susceptibility to schizophrenia. (Letter) Nature Genet. 25: 376-377, 2000.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NOTCH4

Target: NOTCH4 (Notch 4 (NOTCH4))

Alternative Name: NOTCH4

Background:

Synonyms: Neurogenic locus notch homolog protein 4;Notch 4;hNotch4;Notch 4 extracellular truncation;Notch 4 intracellular domain;NOTCH4;INT3;

Tissue Specificity: Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes.

Background: NOTCH4 (NOTCH, DROSOPHILA, HOMOLOG OF, 4) also known as INT3 or NOTCH3, FORMERLY, is a member of the Notch family. In situ hybridization revealed that Notch4 transcripts are primarily restricted to endothelial cells in embryonic and adult life, suggesting a specific role forNotch4 during development of vertebrate endothelium. The sequences of the mouse and human NOTCH4 proteins are 82% identical. Northern blot analysis revealed that NOTCH4 (S) is the major transcript and is expressed in a wide variety of tissues. Fluorescence in situ hybridization confirmed the location of the NOTCH4 gene at chromosome 6p21.3. In linkage disequilibrium mapping of the MHC region in 80 British parent-offspring trios, Wei and Hemmings (2000) found that NOTCH4 was highly associated with schizophrenia. Repression of Notch4 resolved ataxia and reversed the disease progression, demonstrating that Notch4 is not only sufficient to induce but also required to sustain the disease. 

Sequence Similarities: Belongs to the NOTCH family.

Molecular Weight: 210 kDa

UniProt: Q99466

Pathways: Notch Signaling