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NOTCH4 antibody (AA 1261-1273)

Verified NOTCH4 Reactivity: Human, Mouse ELISA, IF Host: Goat Polyclonal unconjugated
Catalog No. ABIN768617
  • Target See all NOTCH4 Antibodies
    NOTCH4 (Notch 4 (NOTCH4))
    Binding Specificity
    • 15
    • 9
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1261-1273
    Reactivity
    • 40
    • 28
    • 20
    • 4
    • 4
    • 4
    • 3
    • 3
    • 1
    • 1
    Human, Mouse
    Host
    • 55
    • 2
    • 2
    Goat
    Clonality
    • 48
    • 11
    Polyclonal
    Conjugate
    • 31
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This NOTCH4 antibody is un-conjugated
    Application
    • 29
    • 16
    • 13
    • 13
    • 6
    • 5
    • 5
    • 4
    • 2
    • 1
    • 1
    ELISA, Immunofluorescence (IF)
    Purpose
    NOTCH4 (aa1261-1273)
    Sequence
    DHFHNGHCEK GCNN
    Specificity
    The immunizong peptide represents part of the extracellular domain.
    Cross-Reactivity
    Cow, Dog, Human, Mouse, Pig, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence DHFHNGHCEKGCNN, from the internal region of the protein sequence according to NP_004548.3.
    Isotype
    IgG
    Top Product
    Discover our top product NOTCH4 Primary Antibody
  • Application Notes

    Western Blot: Preliminary experiments gave an approx 27 kDa band in all human, mouse and rat tissue and cell line lysates tested after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we ob

    Peptide ELISA: antibody detection limit dilution 1:8000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the nuclei and membranes of HeLa cells, and in the membranes of NIH3T3 cells. Recommended concentration: 10μg/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    NOTCH4 (Notch 4 (NOTCH4))
    Alternative Name
    NOTCH4 (NOTCH4 Products)
    Synonyms
    INT3 antibody, NOTCH3 antibody, Int-3 antibody, Int3 antibody, N4 antibody, Notch4 antibody, notch 4 antibody, neurogenic locus notch homolog protein 4 antibody, NOTCH4 antibody, Notch4 antibody, LOC100712850 antibody
    Background
    NOTCH4, DAQB-143M3.4, FLJ16302, INT3, MGC74442, NOTCH3, Notch homolog 4, neurogenic locus notch homolog protein 4
    Molecular Weight
    27kDa
    Gene ID
    4855, 18132, 406162
    NCBI Accession
    NP_004548
    Pathways
    Notch Signaling
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