Notch1 antibody (Middle Region)

Catalog No. ABIN3043031

The Rabbit Polyclonal anti-Notch1 antibody has been validated for WB. It is suitable to detect Notch1 in samples from Human. There is 1 publication available.

Quick Overview for Notch1 antibody (Middle Region) (ABIN3043031)

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Notch1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Notch1 Antibody

Binding Specificity: AA 2487-2506, Middle Region

Purpose: Anti-Notch1 Antibody Picoband®

Sequence: QHSYSSPVDN TPSHQLQVPE

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Notch1 Antibody (ABIN3043031). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Notch1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Conlon, R. A., Reaume, A. G., Rossant, J. : Notch1 is required for the coordinate segmentation of somites. Development 121: 1533-1545, 1995. 2. Tanigaki K, Nogaki F, Takahashi J, Tashiro K, Kurooka H, Honjo T (January 2001). "Notch1 and Notch3 instructively restrict bFGF-responsive multipotent neural progenitor cells to an astroglial fate". Neuron 29 (1): 45-55.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Notch1 antibody (ABIN3043031)

Mi, Chen, Zheng, Li, Zhang, Mo, Yang: "MicroRNA-139-5p Suppresses 3T3-L1 Preadipocyte Differentiation Through Notch and IRS1/PI3K/Akt Insulin Signaling Pathways." in: Journal of cellular biochemistry, Vol. 116, Issue 7, pp. 1195-204, (2015) (PubMed).

Target Details for Notch1

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Alternative Name: NOTCH1

Background:

Synonyms: Neurogenic locus notch homolog protein 1,Notch 1,hN1,Translocation-associated notch protein TAN-1,Notch 1 extracellular truncation,NEXT,Notch 1 intracellular domain,NICD,NOTCH1,TAN1,

Tissue Specificity: In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.

Background: Notch proteins are single-pass transmembrane receptors that regulate cell fate decisions during development. The Notch family includes 4 receptors, NOTCH1, NOTCH2, NOTCH3, and NOTCH4, whose ligands include JAG1, JAG2, DLL1), DLL3, and DLL4. Notch homolog 1, translocation-associated (NOTCH1), is a human gene encoding a single-pass transmembrane receptor. It functions as a receptor for membrane bound ligands, and may play multiple roles during development. NOTCH1 may normally coordinates the process of somitogenesis, and the activated Notch 1 and Notch 3 promote differentiation of progenitor cells into astroglia.

Sequence Similarities: Belongs to the NOTCH family.

Molecular Weight: 272 kDa

UniProt: P46531

Pathways: Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development