CHRNA1 antibody (N-Term)

Catalog No. ABIN3043100

The Rabbit Polyclonal anti-CHRNA1 antibody has been validated for WB. It is suitable to detect CHRNA1 in samples from Human, Mouse and Rat.

Quick Overview for CHRNA1 antibody (N-Term) (ABIN3043100)

Target: CHRNA1 (Acetylcholine Receptor Subunit alpha (CHRNA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRNA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CHRNA1 Antibody

Binding Specificity: AA 22-36, N-Term

Purpose: Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 Antibody Picoband®

Sequence: EHETRLVAKL FKDYS

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 Antibody (ABIN3043100). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Nicotinic Acetylcholine Receptor alpha 1, identical to the related rat sequence, different from the related mouse sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Giraud, M., Taubert, R., Vandiedonck, C., Ke, X., Levi-Strauss, M., Pagani, F., Baralle, F. E., Eymard, B., Tranchant, C., Gajdos, P., Vincent, A., Willcox, N., Beeson, D., Kyewski, B., Garchon, H.-J. : An IRF8-binding promoter variant and AIRE control CHRNA1 promiscuous expression in thymus. Nature 448: 934-937, 2007. 2. Noda, M., Furutani, Y., Takahashi, H., Toyosato, M., Tanabe, T., Shimizu, S., Kikyotani, S., Kayano, T., Hirose, T., Inayama, S., Numa, S. : Cloning and sequence analysis of calf cDNA and human genomic DNA encoding alpha-subunit precursor of muscle acetylcholine receptor. Nature 305: 818-823, 1983.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CHRNA1

Target: CHRNA1 (Acetylcholine Receptor Subunit alpha (CHRNA1))

Alternative Name: CHRNA1

Background:

Synonyms: Acetylcholine receptor subunit alpha,CHRNA1,ACHRA, CHNRA,

Tissue Specificity: Isoform 1 is only expressed in skeletal muscle. Isoform 2 is constitutively expressed in skeletal muscle, brain, heart, kidney, liver, lung and thymus.

Background: CHRNA, also termed ACHRA, is mapped on 2q24-q32.This gene encodes the alpha subunit of the muscle acetylcholine receptor, which is the main target of pathogenic autoantibodies in autoimmune myasthenia gravis. The protein-coding sequence of the human alpha subu gene is divided into 9 exons that correspond to different structural and functional domains of the precursor molecule.

Molecular Weight: 69 kDa

UniProt: P02708

Pathways: Skeletal Muscle Fiber Development