FOXP3 antibody (Middle Region)

Catalog No. ABIN3043158

Cited in 10+ publications. The Rabbit Polyclonal anti-FOXP3 antibody (ABIN3043158) specifically detects FOXP3 in WB. The antibody is reactive with Human samples.

Quick Overview for FOXP3 antibody (Middle Region) (ABIN3043158)

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXP3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FOXP3 Antibody

Binding Specificity: Middle Region

Purpose: Anti-Forkhead box protein P3 FOXP3 Antibody

Sequence: FPEFLHNMDY FKFHNMR

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related mouse sequence by two amino acids.

Characteristics: Anti-Forkhead box protein P3 FOXP3 Antibody. Tested in WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human FOXP3, different from the related mouse sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-FOXP3 antibody (ABIN3043158)

Li, Chen, Xie: "Decreased FOXP3+ and GARP+ Tregs to neoadjuvant chemotherapy associated with favorable prognosis in advanced gastric cancer." in: OncoTargets and therapy, Vol. 9, pp. 3525-33, (2016) (PubMed).

Tao, Zhu, Chen, Wang, Pan, Yu, Zhou, Zhu: "IL-35 improves Treg-mediated immune suppression in atherosclerotic mice." in: Experimental and therapeutic medicine, Vol. 12, Issue 4, pp. 2469-2476, (2016) (PubMed).

Gong, Wang, Yuan, Li, Gu, Zhao, Zhang, Jia, Feng, Liu: "Inhibition of Tumor Growth and Immunomodulatory Effects of Flavonoids and Scutebarbatines of Scutellaria barbata D. Don in Lewis-Bearing C57BL/6 Mice." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 630760, (2015) (PubMed).

Li, Ren, Wang, Gu, Hu, Ren, Hong, Wu, Liu, Li: "T2 enhances in situ level of Foxp3+ regulatory cells and modulates inflammatory cytokines in Crohn's disease." in: International immunopharmacology, Vol. 18, Issue 2, pp. 244-8, (2014) (PubMed).

Wang, Dai, Dong, Sun, Song, Guo, Zhu, Wang, Zhang: "The modulation of endoplasmic reticulum stress by chemical chaperone upregulates immune negative cytokine IL-35 in apolipoprotein E-deficient mice." in: PLoS ONE, Vol. 9, Issue 1, pp. e87787, (2014) (PubMed).

Gai, Li, Song, Lei, Yang: "In situ analysis of FOXP3(+) regulatory T cells and myeloid dendritic cells in human colorectal cancer tissue and tumor-draining lymph node." in: Biomedical reports, Vol. 1, Issue 2, pp. 207-212, (2014) (PubMed).

Hu, Li, Maskey, Xu, Peng, Wang, Li, Yang: "Decreased intratumoral Foxp3 Tregs and increased dendritic cell density by neoadjuvant chemotherapy associated with favorable prognosis in advanced gastric cancer." in: International journal of clinical and experimental pathology, Vol. 7, Issue 8, pp. 4685-94, (2014) (PubMed).

Qiao, Huang, Xu, Zhao, Ran, Shen: "LncRNA DQ786243 affects Treg related CREB and Foxp3 expression in Crohn's disease." in: Journal of biomedical science, Vol. 20, pp. 87, (2014) (PubMed).

Fu, Li, Yang, Gai, Jia, Lei, Li: "FOXP3 and TLR4 protein expression are correlated in non-small cell lung cancer: implications for tumor progression and escape." in: Acta histochemica, Vol. 115, Issue 2, pp. 151-7, (2013) (PubMed).

Gai, Song, Li, Lei, Yang: "Potential role of plasmacytoid dendritic cells for FOXP3+ regulatory T cell development in human colorectal cancer and tumor draining lymph node." in: Pathology, research and practice, Vol. 209, Issue 12, pp. 774-8, (2013) (PubMed).

Target Details for FOXP3

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Alternative Name: FOXP3

Background:

Background: FOXP3 (forkhead box P3) is a protein involved in immune system responses. The human FOXP3 genes contain 11 coding exons. Exon-intron boundaries are identical across the coding regions of the mouse and human genes. By genomic sequence analysis, the FOXP3 gene maps to the p arm of the X chromosome (specifically, Xp11.23). A member of the FOX protein family, FOXP3 appears to function as a master regulator in the development and function of regulatory T cells. While the precise control mechanism has not yet been established, FOX proteins belong to the forkhead/winged-helix family of transcriptional regulators and are presumed to exert control via similar DNA binding interactions during transcription.

Gene Full Name: forkhead box P3

Sequence Similarities: Contains 1 C2H2-type zinc finger.

Molecular Weight: 55 kDa

Gene ID: 50943

UniProt: Q9BZS1

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation