Resistin antibody (C-Term)

Catalog No. ABIN3043196

The Rabbit Polyclonal anti-Resistin antibody has been validated for WB and IHC. It is suitable to detect Resistin in samples from Mouse.

Quick Overview for Resistin antibody (C-Term) (ABIN3043196)

Target: Resistin (RETN)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Resistin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Resistin Antibody

Binding Specificity: AA 93-114, C-Term

Purpose: Anti-Resistin/RETN Antibody Picoband®

Sequence: CHCQCARIDW TAARCCKLQV AS

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Resistin/RETN Antibody (ABIN3043196). Tested in IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse Resistin.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse
Western blot, 0.1-0.5 μg/mL, Mouse
1. Holcomb, I. N., Kabakoff, R. C., Chan, B., Baker, T. W., Gurney, A., Henzel, W., Nelson, C., Lowman, H. B., Wright, B. D., Skelton, N. J., Frantz, G. D., Tumas, D. B., Peale, F. V., Jr., Shelton, D. L., Hebert, C. C.FIZZ1, a novel cysteine-rich secreted protein associated with pulmonary inflammation, defines a new gene family.EMBO J. 19: 4046-4055, 2000. 2. Steppan, C. M., Bailey, S. T., Bhat, S., Brown, E. J., Banerjee, R. R., Wright, C. M., Patel, H. R., Ahima, R. S., Lazar, M. A.The hormone resistin links obesity to diabetes.Nature 409: 307-312, 2001. 3. Wang, H., Chu, W. S., Hemphill, C., Elbein, S. C.Human resistin gene: molecular scanning and evaluation of association with insulin sensitivity and type 2 diabetes in Caucasians.J. Clin. Endocr. Metab. 87: 2520-2524, 2002.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Resistin

Target: Resistin (RETN)

Alternative Name: RETN

Background:

Synonyms: Resistin,Adipose tissue-specific secretory factor,ADSF,Adipose-specific cysteine-rich secreted protein A12-alpha,Cysteine-rich secreted protein FIZZ3,Retn,Fizz3,

Tissue Specificity: Expressed in white but not brown adipose tissue in a variety of organs.

Background: Resistin, also known as adipose tissue-specific secretory factor (ADSF) or C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein (XCP1) is a cysteine-rich protein that in humans is encoded by the RETN gene. The resistin gene comprises 4 exons, the first of which is untranslated, and spans approximately 1,750 bp. The human resistin gene is localized to a cloned fragment of human chromosome 19. In primates, pigs and dogs, resistin is secreted by immune and epithelial cells while in rodents, it is secreted by adipose tissue. Resistin is a cytokine whose physiologic role has been the subject of much controversy regarding its involvement with obesity and type II diabetes mellitus.

Sequence Similarities: Belongs to the cyclophilin-type PPIase family. PPIase D subfamily.

Molecular Weight: 38 kDa

UniProt: Q99P87

Pathways: Feeding Behaviour, Smooth Muscle Cell Migration