IL12B antibody (N-Term)

Catalog No. ABIN3043199

The Rabbit Polyclonal anti-IL12B antibody has been validated for WB. It is suitable to detect IL12B in samples from Human. There is 1 publication available.

Quick Overview for IL12B antibody (N-Term) (ABIN3043199)

Target: IL12B (Interleukin 12 beta (IL12B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL12B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-IL12B Antibody

Binding Specificity: AA 23-40, N-Term

Purpose: Anti-IL12 p40/IL12B Antibody Picoband®

Sequence: IWELKKDVYV VELDWYPD

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-IL12 p40/IL12B Antibody (ABIN3043199). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human IL-12(p40).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Gunsten, S., Mikols, C. L., Grayson, M. H., Schwendener, R. A., Agapov, E., Tidwell, R. M., Cannon, C. L., Brody, S. L., Walter, M. J.IL-12 p80-dependent macrophage recruitment primes the host for increased survival following a lethal respiratory viral infection.Immunology 126: 500-513, 2008. 2. Oppmann, B., Lesley, R., Blom, B., Timans, J. C., Xu, Y., Hunte, B., Vega, F., Yu, N., Wang, J., Singh, K., Zonin, F., Vaisberg, E., and 13 others.Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12.Immunity 13: 715-725, 2000. 3. Sieburth, D., Jabs, E. W., Warrington, J. A., Li, X., Lasota, J., LaForgia, S., Kelleher, K., Huebner, K., Wasmuth, J. J., Wolf, S. F.Assignment of genes encoding a unique cytokine (IL12) composed of two unrelated subunits to chromosomes 3 and 5.Genomics 14: 59-62, 1992.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-IL12B antibody (ABIN3043199)

Dong, Xia, Chen, Chen, Zhang: "Anti-arthritis activity of cationic materials." in: Journal of cellular and molecular medicine, Vol. 14, Issue 7, pp. 2015-24, (2011) (PubMed).

Target Details for IL12B

Target: IL12B (Interleukin 12 beta (IL12B))

Alternative Name: IL12B

Background:

Synonyms: Interleukin-12 subunit beta,IL-12B,Cytotoxic lymphocyte maturation factor 40 kDa subunit,CLMF p40,IL-12 subunit p40,NK cell stimulatory factor chain 2,NKSF2,IL12B,NKSF2,

Background: IL-12, Interleukin 12, heterodimerizes with the IL12 p35 subunit to form IL12 and with the IL23 p19 subunit to form IL23. IL12b p 40 exists as a monomer and as a homodimer. This gene encodes a subunit of Interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The IL-12b gene contains 8 exons, the first and last of which are noncoding. By PCR analysis of DNA from rodent/human cell hybrids containing intact chromosomes or translocation chromosomes, the IL-12b gene is mapped to 5q31-q33.This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen.

Sequence Similarities: Belongs to the type I cytokine receptor family. Type 3 subfamily.

Molecular Weight: 20 kDa

UniProt: P29460

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation