KIF2C antibody (AA 531-725)

Catalog No. ABIN3043278

Product Details anti-KIF2C Antibody

Target: KIF2C (Kinesin Family Member 2C (KIF2C))

Binding Specificity: AA 531-725

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIF2C antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Purpose: Anti-MCAK/KIF2C Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-MCAK/KIF2C Antibody Picoband® (ABIN3043278). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MCAK recombinant protein (Position: G531-Q725). Human MCAK shares 87% amino acid (aa) sequence identity with both mouse and rat MCAK.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Manning, A. L., Ganem, N. J., Bakhoum, S. F., Wagenbach, M., Wordeman, L., Compton, D. A. The kinesin-13 proteins, Kif2a, Kif2b, and Kif2c/MCAK have distinct roles during mitosis in human cells. Molec. Biol. Cell 18: 2970-2979, 2007. 2. Kim IG, Jun DY, Sohn U, Kim YH (Feb 1998). "Cloning and expression of human mitotic centromere-associated kinesin gene". Biochim Biophys Acta 1359 (3): 181-6. 3. "Entrez Gene: KIF2C kinesin family member 2C"

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for KIF2C

Target: KIF2C (Kinesin Family Member 2C (KIF2C))

Alternative Name: KIF2C (KIF2C Products)

Background:

Synonyms: Kinesin-like protein KIF2C,Kinesin-like protein 6,Mitotic centromere-associated kinesin,MCAK,KIF2C,KNSL6,

Tissue Specificity: Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary, expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific. .

Background: Kinesin-like protein KIF2C is a protein that in humans is encoded by the KIF2C gene. It is mapped to 1p34.1. The protein encoded by this gene is a member of kinesin-like protein family. Most proteins of this family are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein acts to regulate microtubule dynamics in cells and is important for anaphase chromosome segregation and may be required to coordinate the onset of sister centromere separation. KIF2C uses microtubule depolymerizing activity to correct improper microtubule attachments at kinetochores.

Sequence Similarities: Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. MCAK/KIF2 subfamily.

Molecular Weight: 81 kDa

Gene ID: 11004

UniProt: Q99661

Pathways: Microtubule Dynamics