Synaptophysin antibody (N-Term)

Catalog No. ABIN3043307

The Rabbit Polyclonal anti-Synaptophysin antibody has been validated for WB. It is suitable to detect Synaptophysin in samples from Human, Rat and Mouse. There are 3+ publications available.

Quick Overview for Synaptophysin antibody (N-Term) (ABIN3043307)

Target: Synaptophysin (SYP)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Synaptophysin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Synaptophysin Antibody

Binding Specificity: AA 1-26, N-Term

Purpose: Anti-Synaptophysin/SYP Antibody Picoband®

Sequence: MLLLADMDVV NQLVAGGQFR VVKEPL

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Synaptophysin/SYP Antibody Picoband® (ABIN3043307). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Synaptophysin, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human
1. Calhoun ME, Jucker M, Martin LJ, Thinakaran G, Price DL, Mouton PR (December 1996). "Comparative evaluation of synaptophysin-based methods for quantification of synapses". J. Neurocytol.25 (12): 821-8.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Synaptophysin antibody (ABIN3043307)

Chen, Wei, Nie, Lin, Tian, Liu, Yu, Cheng, Yan, Wang, Liu, Deng, Lai, Zhou, Zhang, Lin, Chen: "?-Asarone prevents autophagy and synaptic loss by reducing ROCK expression in asenescence-accelerated prone 8 mice." in: Brain research, Vol. 1552, pp. 41-54, (2014) (PubMed).

Liu, Liang, Liu, Zou, Li, Wang, An: "Downregulation of caveolin-1 contributes to the synaptic plasticity deficit in the hippocampus of aged rats." in: Neural regeneration research, Vol. 8, Issue 29, pp. 2725-33, (2014) (PubMed).

Li, Xin, Wang, Wu, Jin, Ma, Nie, Xiao, Hu, Jin: "Pentamethylquercetin protects against diabetes-related cognitive deficits in diabetic Goto-Kakizaki rats." in: Journal of Alzheimer's disease : JAD, Vol. 34, Issue 3, pp. 755-67, (2013) (PubMed).

Target Details for Synaptophysin

Target: Synaptophysin (SYP)

Alternative Name: SYP

Background:

Synonyms: Synaptophysin,Major synaptic vesicle protein p38,SYP,

Tissue Specificity: Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.

Background: SYP (Synaptophysin) is located on the short arm of X chromosome (Xp11.23-p11.22). The protein is a synaptic vesicle glycoprotein with four transmembrane domains weighing 38 kDa. It is present inneuroendocrine cells and in virtually all neurons in the brain and spinal cord that participate in synaptic transmission. It acts as a marker for neuroendocrine tumors, and its ubiquity at the synapse has led to the use of synaptophysin immunostaining for quantification of synapses. Also, the protein binds cholesterol and is thought to targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR).

Sequence Similarities: Belongs to the synaptophysin/synaptobrevin family.

Molecular Weight: 38 kDa

Gene ID: 6855

UniProt: P08247

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity