SERPINB5 antibody (AA 1-350)

Catalog No. ABIN3043330

This Rabbit Polyclonal antibody specifically detects SERPINB5 in WB, IHC, IF and ICC. It exhibits reactivity toward Human and Mouse and has been mentioned in 2+ publications.

Quick Overview for SERPINB5 antibody (AA 1-350) (ABIN3043330)

Target: SERPINB5 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 5 (SERPINB5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SERPINB5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-SERPINB5 Antibody

Binding Specificity: AA 1-350

Purpose: Anti-MASPIN/SERPINB5 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MASPIN/SERPINB5 Antibody Picoband® (ABIN3043330). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MASPIN recombinant protein (Position: M1-A350). Human MASPIN shares 88% and 89% amino acid (aa) sequence identity with mouse and rat MASPIN, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
1. Blandamura S, et al. Maspin expression, subcellular localization and clinicopathological correlation in endometrial hyperplasia and endometrial adenocarcinoma. Histol Histopathol, 2014 Jun. 2. Taskiran C, et al. Maspin expression in endometrial hyperplasia and carcinoma, and its relation with angiogenesis. Eur J Gynaecol Oncol, 2014. 3. Triulzi T, et al. Maspin influences response to doxorubicin by changing the tumor microenvironment organization. Int J Cancer, 2014 Jun 15.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-SERPINB5 antibody (ABIN3043330)

Zhang, Qi, Cao, Zu, Chen: "Expression and clinical significance of microRNA-21, maspin and vascular endothelial growth factor-C in bladder cancer." in: Oncology letters, Vol. 10, Issue 4, pp. 2610-2616, (2015) (PubMed).

Yang, Shi, Li, Yi: "Effects of shRNA targeting maspin on invasion of gastric carcinoma SGC7901 cell line." in: Oncology reports, Vol. 25, Issue 1, pp. 259-65, (2010) (PubMed).

Target Details for SERPINB5

Target: SERPINB5 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 5 (SERPINB5))

Alternative Name: SERPINB5

Background:

Synonyms: Serpin B5,Maspin,Peptidase inhibitor 5,PI-5,SERPINB5,PI5,

Tissue Specificity: Normal mammary epithelial cells.

Background: SERPINB5 is also known as PI5 or maspin. Maspin (mammary serine protease inhibitor) is a protein that in humans is encoded by the SERPINB5 gene. Maspin is expressed in the skin, prostate, testis, intestine, tongue, lung, and the thymus. Maspin is a member of the serpin superfamily of serine protease inhibitors.[1] The primary function of most members of this family is to regulate the breakdown of proteins by inhibiting the catalytic activity of proteinases. Through this mechanism of action, serpins regulate a number of cellular processes includingphagocytosis, coagulation, and fibrinolysis.

Sequence Similarities: Belongs to the serpin family. Ov-serpin subfamily.

Molecular Weight: 42 kDa

Gene ID: 5268

UniProt: P36952

Pathways: p53 Signaling