SERPINB5
Reactivity: Human
WB, IHC, ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Sandwich Elisa: To detect Human Maspin by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with Biotinylated Anti-Human Maspin (ABIN487957 orABIN487958) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant Human Maspin. Western Blot: To detect Human Maspin by Western Blot analysis this antibody can be usedat a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human Maspin is 1.5-3.0 ng/lane, under eitherreducing or non-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2 without preservatives.
Preservative
Without preservative
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody prior to reconstitution at-20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at-20 °C for longer.
Target
SERPINB5
(serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 5 (SERPINB5))
1110036M19Rik antibody, AI462524 antibody, AI646751 antibody, Maspin antibody, PI-5 antibody, Spi7 antibody, ovalbumin antibody, PI5 antibody, maspin antibody, Pi5 antibody, serine (or cysteine) peptidase inhibitor, clade B, member 5 antibody, serpin family B member 5 antibody, serpin peptidase inhibitor, clade B (ovalbumin), member 5 S homeolog antibody, Serpinb5 antibody, SERPINB5 antibody, serpinb5.S antibody
Background
MASPIN is a tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.Synonyms: PI-5, PI5, Peptidase inhibitor 5, Protease inhibitor 5, Serpin B5