SIRT7 antibody (AA 8-350)

Catalog No. ABIN3043336

This Rabbit Polyclonal antibody specifically detects SIRT7 in WB and IHC. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for SIRT7 antibody (AA 8-350) (ABIN3043336)

Target: SIRT7 (Sirtuin 7 (SIRT7))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SIRT7 Antibody

Binding Specificity: AA 8-350

Purpose: Anti-SIRT7 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SIRT7 Antibody Picoband® (ABIN3043336). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SIRT7 recombinant protein (Position: R8-G350). Human SIRT7 shares 96% and 95% amino acid (aa) sequence identity with mouse and rat SIRT7, respectively.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat, Human
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Altered sirtuin expression is associated with node-positive breast cancer.Ashraf N, et al. Br J Cancer, 2006 Oct 23. 2. Molecular pathways: emerging roles of mammalian Sirtuin SIRT7 in cancer.Paredes S, et al. Clin Cancer Res, 2014 Apr 1. 3. Sirtuin 7 plays a role in ribosome biogenesis and protein synthesis.Tsai YC, et al. Mol Cell Proteomics, 2014 Jan.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SIRT7

Target: SIRT7 (Sirtuin 7 (SIRT7))

Alternative Name: SIRT7

Background:

Synonyms: NAD-dependent protein deacetylase sirtuin-7,3.5.1.-,Regulatory protein SIR2 homolog 7,SIR2-like protein 7,SIRT7,SIR2L7,

Background: SIRT7 is also known as SIR2L7. This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined, however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.

Sequence Similarities: Belongs to the sirtuin family. Class IV subfamily.

Molecular Weight: 40 kDa

Gene ID: 51547