SLC10A1 antibody (C-Term)

Catalog No. ABIN3043337

The Rabbit Polyclonal anti-SLC10A1 antibody is suitable to detect SLC10A1 in samples from Rat and Mouse. It has been validated for WB, FACS, IHC, IF and IHC (fro).

Quick Overview for SLC10A1 antibody (C-Term) (ABIN3043337)

Target: SLC10A1 (Solute Carrier Family 10 (Sodium/bile Acid Cotransporter Family), Member 1 (SLC10A1))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC10A1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SLC10A1 Antibody

Binding Specificity: AA 296-336, C-Term

Purpose: Anti-SLC10A1 Antibody

Sequence: EGLLFIIIFR CYLKIKPQKD QTKITYKAAA TEDATPAALE K

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SLC10A1 Antibody (ABIN3043337). Tested in Flow Cytometry, IF, IHC, IHC-F, WB applications. This antibody reacts with Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse SLC10A1, different from the related human sequence by eighteen amino acids, and from the related rat sequence by three amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SLC10A1

Target: SLC10A1 (Solute Carrier Family 10 (Sodium/bile Acid Cotransporter Family), Member 1 (SLC10A1))

Alternative Name: Slc10a1

Background:

Synonyms: Sodium/bile acid cotransporter,Na (+)/bile acid cotransporter,Na (+)/taurocholate transport protein,Sodium/taurocholate cotransporting polypeptide,Solute carrier family 10 member 1,Slc10a1,Ntcp,

Tissue Specificity: Predominantly expressed in the brain.

Background: Na+-taurocholate cotransporting polypeptide (NTCP), also known as SLC10A1 (Solute carrier family 10, member 1), is the major bile acid uptake system in human hepatocytes. NTCP and the ileal transporter ASBT (apical sodium-dependent bile acid transporter) are two sodium-dependent transporters critical for the enterohepatic circulation of bile acids. The hASBT gene is known to be activated by the glucocorticoid receptor (GR). Ho RH et al. indicates functionally important polymorphisms in NTCP exist and that the like lihood of being carriers of such polymorphisms is dependent on ethnicity.

Molecular Weight: 50 kDa

Gene ID: 20493

UniProt: O08705