MTDH antibody (AA 101-270)

Catalog No. ABIN3043367

The Rabbit Polyclonal anti-MTDH antibody has been validated for WB, IHC and ICC. It is suitable to detect MTDH in samples from Human, Mouse and Rat.

Quick Overview for MTDH antibody (AA 101-270) (ABIN3043367)

Target: MTDH (Metadherin (MTDH))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MTDH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-MTDH Antibody

Binding Specificity: AA 101-270

Purpose: Anti-LYRIC/MTDH Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-LYRIC/MTDH Antibody Picoband® (ABIN3043367). Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human LYRIC recombinant protein (Position: D101-Q270). Human LYRIC shares 94% amino acid (aa) sequence identity with both mouse and rat LYRIC.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Western blot, 0.1-0.5 μg/mL
1. Brown DM, Ruoslahti E (April 2004). "Metadherin, a cell surface protein in breast tumors that mediates lung metastasis". Cancer Cell 5 (4): 365-74. 2. Hu G, Chong RA, Yang Q, Wei Y, Blanco MA, Li F, Reiss M, Au JL, Haffty BG, Kang Y (January 2009). "MTDH activation by 8q22 genomic gain promotes chemoresistance and metastasis of poor-prognosis breast cancer". Cancer Cell 15 (1): 9-20. 3. Yoo BK, Emdad L, Lee SG, Su ZZ, Santhekadur P, Chen D, Gredler R, Fisher PB, Sarkar D (January 2011)."Astrocyte elevated gene-1 (AEG-1): A multifunctional regulator of normal and abnormal physiology".Pharmacol Ther 130 (1): 1-8.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MTDH

Target: MTDH (Metadherin (MTDH))

Alternative Name: MTDH

Background:

Synonyms: Protein LYRIC,3D3/LYRIC,Astrocyte elevated gene-1 protein,AEG-1,Lysine-rich CEACAM1 co-isolated protein,Metadherin,Metastasis adhesion protein,MTDH,AEG1, LYRIC,

Tissue Specificity: Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme. .

Background: MTDH (Metadherin), also known as protein LYRIC or astrocyte elevated gene-1 protein (AEG-1) is a protein that in humans is encoded by the MTDH gene. AEG-1 is involved in HIF-1alpha mediated angiogenesis. AEG-1 also interacts with SND1 and involved in RNA-induced silencing complex (RISC) and plays very important role in RISC and miRNA functions. AEG-1 induces an oncogene called Late SV40 factor (LSF/TFCP2) which is involved in thymidylate synthase (TS) induction and DNA biosynthesis synthesis. Late SV40 factor (LSF/TFCP2) enhances angiogenesis by transcriptionally up-regulating matrix metalloproteinase-9 (MMP9). AEG-1 acts as an oncogene in melanoma, malignant glioma, breast cancer and hepatocellular carcinoma. It is highly expressed in these cancers and helps in progression and development of these cancers. It is induced by c-Myc oncogene and plays very important role in anchorage independent growth of cancer cells.

Sequence Similarities: Belongs to the chaperonin (HSP60) family.

Molecular Weight: 70-80 kDa

Gene ID: 92140

Pathways: Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization