RBP4 antibody (AA 19-201)

Catalog No. ABIN3043369

This Rabbit Polyclonal antibody specifically detects RBP4 in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RBP4 antibody (AA 19-201) (ABIN3043369)

Target: RBP4 (Retinol Binding Protein 4, Plasma (RBP4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RBP4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-RBP4 Antibody

Binding Specificity: AA 19-201

Purpose: Anti-RBP4 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RBP4 Antibody Picoband® (ABIN3043369). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RBP4 recombinant protein (Position: E19-201L). Human RBP4 shares 86% amino acid (aa) sequence identity with both mouse and rat RBP4.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Cubedo J, et al. Retinol-binding protein 4 levels and susceptibility to ischaemic events in men. Eur J Clin Invest, 2014. 2. Li F, et al. Retinol-binding protein 4 as a novel risk factor for cardiovascular disease in patients with coronary artery disease and hyperinsulinemia. Am J Med Sci, 2014 Dec. 3. Saucedo R, et al. RBP4 gene variants are associated with insulin resistance in women with previous gestational diabetes. Dis Markers, 2014.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for RBP4

Target: RBP4 (Retinol Binding Protein 4, Plasma (RBP4))

Alternative Name: RBP4

Background:

Synonyms: Retinol-binding protein 4,Plasma retinol-binding protein,PRBP,RBP,Plasma retinol-binding protein (1-182),Plasma retinol-binding protein (1-181),Plasma retinol-binding protein (1-179),Plasma retinol-binding protein (1-176),RBP4,PRO2222,

Tissue Specificity: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines. .

Background: RBP4 is also known as RDCCAS. This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells.

Sequence Similarities: Belongs to the calycin superfamily. Lipocalin family.

Molecular Weight: 23 kDa

Gene ID: 5950

UniProt: P02753

Pathways: Regulatory RNA Pathways, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response