EED antibody (AA 256-441)

Catalog No. ABIN3043370

The Rabbit Polyclonal anti-EED antibody has been validated for WB. It is suitable to detect EED in samples from Human and Rat.

Quick Overview for EED antibody (AA 256-441) (ABIN3043370)

Target: EED (Embryonic Ectoderm Development (EED))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EED antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EED Antibody

Binding Specificity: AA 256-441

Purpose: Anti-EED Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-EED Antibody Picoband® (ABIN3043370). Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human EED recombinant protein (Position: M256-R441). Human EED shares 100% amino acid (aa) sequence identity with mouse EED.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat
1. "Entrez Gene: EED embryonic ectoderm development". 2. Rietzler M, Bittner M, Kolanus W, Schuster A, Holzmann B (Nov 1998). "The human WD repeat protein WAIT-1 specifically interacts with the cytoplasmic tails of beta7-integrins". J. Biol. Chem. 273 (42): 27459-66. 3. Schumacher A, Lichtarge O, Schwartz S, Magnuson T (Jan 1999). "The murine Polycomb-group gene eed and its human orthologue: functional implications of evolutionary conservation". Genomics 54(1): 79-88.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for EED

Target: EED (Embryonic Ectoderm Development (EED))

Alternative Name: EED

Background:

Synonyms: Polycomb protein EED,hEED,WD protein associating with integrin cytoplasmic tails 1,WAIT-1,EED,

Tissue Specificity: Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer. .

Background: Polycomb protein EED is a protein that in humans is encoded by the EED gene. It is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin β7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. Furthermore, this protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene.

Sequence Similarities: Belongs to the WD repeat ESC family.

Molecular Weight: 72 kDa

Gene ID: 8726

UniProt: O75530