NUMB antibody (N-Term)

Catalog No. ABIN3043374

This anti-NUMB antibody is a Rabbit Polyclonal antibody detecting NUMB in WB, IHC and FACS. Suitable for Human, Mouse, Rat and Monkey.

Quick Overview for NUMB antibody (N-Term) (ABIN3043374)

Target: NUMB (Numb Homolog (NUMB))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUMB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-NUMB Antibody

Binding Specificity: AA 194-220, N-Term

Purpose: Anti-NUMB Antibody Picoband®

Sequence: SFRVTTATEQ AEREEIMKQM QDAKKAE

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-NUMB Antibody Picoband® (ABIN3043374). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human NUMB, different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Dho SE, French MB, Woods SA, McGlade CJ (November 1999). "Characterization of four mammalian numb protein isoforms. Identification of cytoplasmic and membrane-associated variants of the phosphotyrosine binding domain". J. Biol. Chem. 274 (46): 33097-104. 2. Katoh M, Katoh M (September 2006). "NUMB is a break of WNT-Notch signaling cycle". Int. J. Mol. Med. 18(3): 517-21. 3. Pece S, Confalonieri S, R Romano P, Di Fiore PP (January 2011). "NUMB-ing down cancer by more than just a NOTCH". Biochim. Biophys. Acta 1815 (1): 26-43.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for NUMB

Target: NUMB (Numb Homolog (NUMB))

Alternative Name: NUMB

Background:

Synonyms: Protein numb homolog,h-Numb,Protein S171,NUMB,

Tissue Specificity: The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta, moderately in lung, liver, skeletal muscle, kidney and pancreas, and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.

Background: Protein numb homolog is a protein that in humans is encoded by the NUMB gene. It is mapped to 14q24.2 to q24.3. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The primary function of Numb in cell differentiation is as an inhibitor of Notch signaling which is essential for maintaining self-renewal potential in stem and progenitor cells. Numb also plays a crucial role in asymmetrical cell division during development, allowing for differential cell fate specification in the central and peripheral nervous systems. What's more, the numb gene protein product controls binary cell fate decisions in the peripheral and central nervous systems of both invertebrates and mammals during neurogenesis.

Sequence Similarities: Contains 1 PID domain.

Molecular Weight: 71 kDa

Gene ID: 8650

UniProt: P49757

Pathways: Cell-Cell Junction Organization