PAX6 antibody (Middle Region)

Catalog No. ABIN3043379

The Rabbit Polyclonal anti-PAX6 antibody has been validated for WB and IHC. It is suitable to detect PAX6 in samples from Human, Mouse and Rat. There is 1 publication available.

Quick Overview for PAX6 antibody (Middle Region) (ABIN3043379)

Target: PAX6 (Paired Box 6 (PAX6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAX6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PAX6 Antibody

Binding Specificity: AA 195-233, Middle Region

Purpose: Anti-PAX6 Antibody Picoband®

Sequence: EDSDEAQMRL QLKRKLQRNR TSFTQEQIEA LEKEFERTH

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PAX6 Antibody Picoband® (ABIN3043379). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PAX6, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Gehring WJ, Ikeo K (September 1999). "Pax 6: mastering eye morphogenesis and eye evolution". Trends Genet. 15 (9): 371-7. 2. Jordan T, Hanson I, Zaletayev D, Hodgson S, Prosser J, Seawright A, Hastie N, van Heyningen V (August 1992). "The human PAX6 gene is mutated in two patients with aniridia". Nat. Genet. 1 (5): 328-32. 3. Walcher T, Xie Q, Sun J, Irmler M, Beckers J, 79ztürk T, Niessing D, Stoykova A, Cvekl A, Ninkovic J, Götz M (March 2013). "Functional dissection of the paired domain of Pax6 reveals molecular mechanisms of coordinating neurogenesis and proliferation". Development 140 (5): 1123-36.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-PAX6 antibody (ABIN3043379)

Liu, Novak, Pepin, Maschhoff, Worner, Chen, Zhang, Hu: "A congenital hydrocephalus-causing mutation in Trim71 induces stem cell defects via inhibiting Lsd1 mRNA translation." in: EMBO reports, Vol. 24, Issue 2, pp. e55843, (2023) (PubMed).

Target Details for PAX6

Target: PAX6 (Paired Box 6 (PAX6))

Alternative Name: PAX6

Background:

Synonyms: Paired box protein Pax-6,Aniridia type II protein,Oculorhombin,PAX6,AN2,

Tissue Specificity: Fetal eye, brain, spinal cord and olfactory epithelium. Isoform 5a is less abundant than the PAX6 shorter form.

Background: Paired box protein Pax-6, also known as aniridia type II protein (AN2) or oculorhombin, is a protein that in humans is encoded by the PAX6 gene. This gene encodes a homeobox and paired domain-containing protein that binds DNA and functions as a regulator of transcription. Activity of this protein is key in the development of neural tissues, particularly the eye. In addition, this gene is regulated by multiple enhancers located up to hundreds of kilobases distant from this locus. Mutations in this gene or in the enhancer regions can cause ocular disorders such as aniridia and Peter's anomaly. Use of alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms.

Molecular Weight: 47 kDa

Gene ID: 5080

UniProt: P26367

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis