CD44 antibody (N-Term)

Catalog No. ABIN3043394

Product Details anti-CD44 Antibody

Target: CD44

Binding Specificity: AA 29-56, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for CD44 antigen(CD44) detection. Tested with WB, IHC-P in Human.

Sequence: RFAGVFHVEK NGRYSISRTE AADLCKAF

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for CD44 antigen(CD44) detection. Tested with WB, IHC-P in Human.
Gene Name: CD44 Molecule (Indian blood group)
Protein Name: CD44 antigen

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human CD44(29-56aa RFAGVFHVEKNGRYSISRTEAADLCKAF), different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-CD44 antibody (ABIN3043394)

Zhang, Yan, Liu, Li, Zhang, Pang, Hu, Zhao, Xie, Zhou, Wang, Xie: "Smad3-related miRNAs regulated oncogenic TRIB2 promoter activity to effectively suppress lung adenocarcinoma growth." in: Cell death & disease, Vol. 7, Issue 12, pp. e2528, (2017) (PubMed).

Ding, Chang, Niu, Dai, Geng, Li, Guo, Xu: "Overexpression of transcription factor Foxa2 and Hnf1α induced rat bone mesenchymal stem cells into hepatocytes." in: Cytotechnology, Vol. 68, Issue 5, pp. 2037-47, (2016) (PubMed).

Zhang, Shang, Hao, Zheng, Li, Liang, Cui, Liu: "Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats." in: American journal of translational research, Vol. 7, Issue 11, pp. 2176-86, (2016) (PubMed).

Zhang, Wang, Dai, Cai, Zhan, Gang, Hu, Xia, Zhang: "Lentivirus-mediated PLCγ1 gene short-hairpin RNA suppresses tumor growth and metastasis of human gastric adenocarcinoma." in: Oncotarget, Vol. 7, Issue 7, pp. 8043-54, (2016) (PubMed).

Yin, Zhou, Wang, Ma, Li, Wang, She, Li: "CD44 promotes the migration of bone marrow-derived mesenchymal stem cells toward glioma." in: Oncology letters, Vol. 11, Issue 4, pp. 2353-2358, (2016) (PubMed).

Dai, Chen, Ding, Niu, Fan, Xu: "Induction of Functional Hepatocyte-Like Cells by Overexpression of FOXA3 and HNF4? in Rat Bone Marrow Mesenchymal Stem Cells." in: Cells, tissues, organs, Vol. 200, Issue 2, pp. 132-40, (2015) (PubMed).

Yuan, Liu, Li, Li, Sun, Xu, Man, Fu: "Effects of BMSCs interactions with adventitial fibroblasts in transdifferentiation and ultrastructure processes." in: International journal of clinical and experimental pathology, Vol. 7, Issue 7, pp. 3957-65, (2015) (PubMed).

Shi, Han, Deng, Zhang, Shen, Gong, Zhang, Sun: "Dual drugs (microRNA-34a and paclitaxel)-loaded functional solid lipid nanoparticles for synergistic cancer cell suppression." in: Journal of controlled release : official journal of the Controlled Release Society, Vol. 194, pp. 228-37, (2014) (PubMed).

Li, Huang, Zheng, Liu, Niu, Huang, Zhao, Fan: "Effect of substrate stiffness on the functions of rat bone marrow and adipose tissue derived mesenchymal stem cells in vitro." in: Journal of biomedical materials research. Part A, Vol. 102, Issue 4, pp. 1092-101, (2014) (PubMed).

Li, Liu, Mei, Chen, Liu, Zhao: "Overexpression of inducible nitric oxide synthase impairs the survival of bone marrow stem cells transplanted into rat infarcted myocardium." in: Life sciences, Vol. 106, Issue 1-2, pp. 50-7, (2014) (PubMed).

Du, Liu, Dang, Zhu, Su, Fan, Tan, Wang, Fang: "Comparison of administration routes for adipose-derived stem cells in the treatment of middle cerebral artery occlusion in rats." in: Acta histochemica, Vol. 116, Issue 6, pp. 1075-84, (2014) (PubMed).

Hefei, Yu, Haiqing, Xiao, Jingyuan, Dongjun: "Morphological Characteristics and Identification of Islet-like Cells Derived From Rat Adipose-derived Stem Cells Cocultured with Pancreas Adult Stem Cells." in: Cell biology international, (2014) (PubMed).

Fu, Lv, Zhuang, Chen, Lv, Li, Wang: "An effective inducer of dopaminergic neuron-like differentiation." in: Neural regeneration research, Vol. 8, Issue 5, pp. 427-34, (2014) (PubMed).

Lin, Li, Han, Li, Lu, Bai, Chen: "Folium Sennae protects against hydroxyl radical-induced DNA damage via antioxidant mechanism: an in vitro study." in: Botanical studies, Vol. 55, Issue 1, pp. 16, (2014) (PubMed).

Dai, Li, Zhou, Chen, Chen, Xiao: "Genistein promotion of osteogenic differentiation through BMP2/SMAD5/RUNX2 signaling." in: International journal of biological sciences, Vol. 9, Issue 10, pp. 1089-98, (2013) (PubMed).

Hou, Zou, Ge, Shen, Wang: "The critical role of CD133(+)CD44(+/high) tumor cells in hematogenous metastasis of liver cancers." in: Cell research, Vol. 22, Issue 1, pp. 259-72, (2012) (PubMed).

Han, Liu, Wang, Mo, Bi, Liu, Fan, Chen, Wu: "Re-expression of miR-21 contributes to migration and invasion by inducing epithelial-mesenchymal transition consistent with cancer stem cell characteristics in MCF-7 cells." in: Molecular and cellular biochemistry, Vol. 363, Issue 1-2, pp. 427-36, (2012) (PubMed).

Han, Liu, Wang, Chen, Xu, Sun, Zhao, Qu, Fan, Wu: "Antagonism of miR-21 reverses epithelial-mesenchymal transition and cancer stem cell phenotype through AKT/ERK1/2 inactivation by targeting PTEN." in: PLoS ONE, Vol. 7, Issue 6, pp. e39520, (2012) (PubMed).

Huang, Chang, Yang, Wu: "Mineralized and osteoid tissue from dental pulp stem cells on micro-arc oxidation titanium in vitro." in: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 32, Issue 4, pp. 620-5, (2012) (PubMed).

Huang, Yuan, Yang: "Effects of total flavonoids from Drynaria fortunei on the proliferation and osteogenic differentiation of rat dental pulp stem cells." in: Molecular medicine reports, Vol. 6, Issue 3, pp. 547-52, (2012) (PubMed).

Target Details for CD44

Target: CD44

Alternative Name: CD44 (CD44 Products)

Synonyms: CDW44 antibody, CSPG8 antibody, ECMR-III antibody, HCELL antibody, HUTCH-I antibody, IN antibody, LHR antibody, MC56 antibody, MDU2 antibody, MDU3 antibody, MIC4 antibody, Pgp1 antibody, AU023126 antibody, AW121933 antibody, AW146109 antibody, HERMES antibody, Ly-24 antibody, Pgp-1 antibody, CD44A antibody, METAA antibody, RHAMM antibody, XCD44 antibody, cd44 antibody, cdw44 antibody, cspg8 antibody, ecmr-iii antibody, hcell antibody, lhr antibody, mc56 antibody, mdu2 antibody, mdu3 antibody, mic4 antibody, mutch-i antibody, pgp1 antibody, CD44 molecule (Indian blood group) antibody, syndecan 4 antibody, CD44 antigen antibody, CD44 molecule (Indian blood group) S homeolog antibody, CD44 antibody, SDC4 antibody, Cd44 antibody, cd44.S antibody

Background: CD44 is also known as LHR or MC56. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

Synonyms: LHR antibody|BA-1 antibody|CD 44 antibody|CD44 antibody|CD44 antigen antibody|CD44 isoform RC antibody|CD44 Molecule (Indian blood group) antibody|CD44 Molecule antibody|CD44_HUMAN antibody|CDw44 antibody|Cell surface glycoprotein CD44 antibody|chondroitin sulfate proteoglycan 8 antibody|CSPG8 antibody|ECM III antibody|ECMR III antibody|ECMR-III antibody|ECMRIII antibody|Epican antibody|Extracellular Matrix Receptor III antibody|GP90 Lymphocyte Homing Adhesion Receptor antibody|GP90 lymphocyte homing/adhesion receptor antibody|HCAM antibody|HCELL antibody|hematopoietic cell E- and L-selectin ligand antibody|Heparan Sulfate Proteoglycan antibody|Hermes Antigen antibody|homing function and Indian blood group system antibody|HSA antibody|HUTCH I antibody|HUTCH-I antibody|HUTCH1 antibody|Hyaluronate Receptor antibody|IN antibody|Indian blood group antibody|Inlu Related p80 Glycoprotein antibody|lhr antibody|Ly 24 antibody|MC56 antibody|MDU2 antibody|MDU3 antibody|MGC10468 antibody|MIC4 antibody|MUTCH I antibody|MUTCH1 antibody|PGP 1 antibody|PGP-1 antibody|PGP-I antibody|PGP1 antibody|Phagocytic Glycoprotein 1 antibody|Phagocytic glycoprotein I antibody|Transmembrane glycoprotein antibody

Gene ID: 960

UniProt: P16070

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling