MMP12 antibody (C-Term)

Catalog No. ABIN3043407

This anti-MMP12 antibody is a Rabbit Polyclonal antibody detecting MMP12 in WB and ELISA. Suitable for Mouse.

Quick Overview for MMP12 antibody (C-Term) (ABIN3043407)

Target: MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP12 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MMP12 Antibody

Binding Specificity: AA 432-466, C-Term

Purpose: Anti-Macrophage metalloelastase MMP12 Antibody Picoband®

Sequence: KIDAVLYFKR HYYIFQGAYQ LEYDPLFRRV TKTLK

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Macrophage metalloelastase MMP12 Antibody (ABIN3043407). Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse MMP12, different from the related human sequence by thirteen amino acids, and from the related rat sequence by three amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, -
ELISA, 0.1-0.5 μg/mL, -
1. Houghton, A. M., Hartzell, W. O., Robbins, C. S., Gomis-Ruth, F. X., Shapiro, S. D.Macrophage elastase kills bacteria within murine macrophages. Nature 460: 637-641, 2009. 2. Joos, L., He, J.-Q., Shepherdson, M. B., Connett, J. E., Anthonisen, N. R., Pare, P. D., Sandford, A. J. The role of matrix metalloproteinase polymorphisms in the rate of decline in lung function. Hum. Molec. Genet. 11: 569-576, 2002. Note: Erratum: Hum. Molec. Genet. 12: 803-804, 2003. 3. Wu, L., Tanimoto, A., Murata, Y., Sasaguri, T., Fan, J., Sasaguri, Y., Watanabe, T. Matrix metalloproteinase-12 gene expression in human vascular smooth muscle cells.Genes Cells 8: 225-234, 2003.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MMP12

Target: MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))

Alternative Name: Mmp12

Background:

Synonyms: Macrophage metalloelastase,MME,3.4.24.65,Matrix metalloproteinase-12,MMP-12,Mmp12,Mme, Mmel,

Background: Matrix metalloproteinase-12 (MMP12), also known as MME or ME, is an enzyme that in humans is encoded by the MMP12 gene. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.2. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. This gene may involved in tissue injury and remodeling.

Molecular Weight: 55 kDa

Gene ID: 17381

UniProt: P34960