EIF4A2 antibody (N-Term)

Catalog No. ABIN3043426

This Rabbit Polyclonal antibody specifically detects EIF4A2 in WB, IHC, FACS, IF, IP, IHC (fro) and ICC. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for EIF4A2 antibody (N-Term) (ABIN3043426)

Target: EIF4A2 (Eukaryotic Translation Initiation Factor 4A2 (EIF4A2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Product Details anti-EIF4A2 Antibody

Binding Specificity: N-Term

Purpose: Anti-eIF4A2 Antibody

Sequence: SADYNREHGG PEGMDPDGVI ESNWNEI

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: identical to the related mouse,rat sequences.

Characteristics: Anti-eIF4A2 Antibody. Tested in Flow Cytometry, IP, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human eIF4A2, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Immunoprecipitation, 0.5-2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for EIF4A2

Target: EIF4A2 (Eukaryotic Translation Initiation Factor 4A2 (EIF4A2))

Alternative Name: EIF4A2

Background:

Background: Eukaryotic initiation factor 4A-II is a protein that in humans is encoded by the EIF4A2 gene. It is mapped to 18p11.2. Eukaryotic initiation factor 4A plays an important role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins. Two highly homologous forms of functional EIF4A genes, Eif4a1 and Eif4a2, have been isolated in mice, yeast cells also possess 2 EIF4A genes, TIF1 and TIF2. The murine Eif4a and yeast TIF genes appear to belong to a DEAD-box gene family, whose members exhibit extensive amino acid similarity and contain the asp-glu-ala-asp (DEAD) sequence. DEAD-box genes have been identified in species ranging from E-coli to humans. Their function appears to be related to transcriptional/translational regulation.

Gene Full Name: eukaryotic translation initiation factor 4A2

Molecular Weight: 49 kDa

Gene ID: 1974

UniProt: Q14240