RUNX3 antibody (AA 128-270)

Catalog No. ABIN3043429

This Rabbit Polyclonal antibody specifically detects RUNX3 in WB, IHC, IF, FACS and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RUNX3 antibody (AA 128-270) (ABIN3043429)

Target: RUNX3 (Runt-Related Transcription Factor 3 (RUNX3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUNX3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-RUNX3 Antibody

Binding Specificity: AA 128-270

Purpose: Anti-RUNX3 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human RUNX3 shares 93% amino acid (aa) sequence identity with mouse RUNX3.

Characteristics: Anti-RUNX3 Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RUNX3 recombinant protein (Position: M128-Y270). Human RUNX3 shares 93% amino acid (aa) sequence identity with mouse RUNX3.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for RUNX3

Target: RUNX3 (Runt-Related Transcription Factor 3 (RUNX3))

Alternative Name: RUNX3

Background:

Background: Runt-related transcription factor 3, also called AML2 is a protein that in humans is encoded by the RUNX3 gene. The RUNX3 gene encodes a Runt-related transcription factor, which is part of the RUNX gene family. By fluorescence in situ hybridization, RUNX3 was assigned to human chromosome 1p36.11. RUNX3 binds to the core site of murine Leukemia virus, the core sequences in the enhancer of the polyomavirus, and also to the enhancers of the T-cell receptor genes. It may be involved in the control of cellular proliferation and/or differentiation.

Gene Full Name: RUNX family transcription factor 3

Sequence Similarities: Belongs to the small GTPase superfamily. Rho family.

Molecular Weight: 43-48 kDa

Gene ID: 864

UniProt: Q13761