SGCA antibody (AA 24-387)

Catalog No. ABIN3043432

This Rabbit Polyclonal antibody specifically detects SGCA in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SGCA antibody (AA 24-387) (ABIN3043432)

Target: SGCA (Sarcoglycan, alpha (50kDa Dystrophin-Associated Glycoprotein) (SGCA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SGCA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SGCA Antibody

Binding Specificity: AA 24-387

Purpose: Anti-alpha Sarcoglycan/SGCA Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-alpha Sarcoglycan/SGCA Antibody Picoband® (ABIN3043432). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SGCA recombinant protein (Position: Q24-H387). Human SGCA shares 90.7% amino acid (aa) sequence identity with mouse SGCA.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human
1. "Entrez Gene: SGCA sarcoglycan, alpha (50 kDa dystrophin-associated glycoprotein)". 2. McNally EM, Yoshida M, Mizuno Y, Ozawa E, Kunkel LM (Nov 1994). "Human adhalin is alternatively spliced and the gene is located on chromosome 17q21". Proc Natl Acad Sci U S A 91 (21): 9690-4.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SGCA

Target: SGCA (Sarcoglycan, alpha (50kDa Dystrophin-Associated Glycoprotein) (SGCA))

Alternative Name: SGCA

Background:

Synonyms: Alpha-sarcoglycan,Alpha-SG,50 kDa dystrophin-associated glycoprotein,50DAG,Adhalin,Dystroglycan-2,SGCA,ADL, DAG2,

Tissue Specificity: Most strongly expressed in skeletal muscle. Also expressed in cardiac muscle and, at much lower levels, in lung. In the fetus, most abundant in cardiac muscle and, at lower levels, in lung. Also detected in liver and kidney. Not expressed in brain.

Background: Alpha-sarcoglycan is a protein that in humans is encoded by the SGCA gene. This gene encodes a component of the dystrophin-glycoprotein complex (DGC), which is critical to the stability of muscle fiber membranes and to the linking of the actin cytoskeleton to the extracellular matrix. Its expression is thought to be restricted to striated muscle. Mutations in this gene result in type 2D autosomal recessive limb-girdle muscular dystrophy. Multiple transcript variants encoding different isoforms have been found for this gene.

Sequence Similarities: Belongs to the sarcoglycan alpha/epsilon family.

Molecular Weight: 43 kDa

Gene ID: 6442

UniProt: Q16586