PRC1 antibody (AA 1-233)

Catalog No. ABIN3043445

The Rabbit Polyclonal anti-PRC1 antibody has been validated for WB. It is suitable to detect PRC1 in samples from Human.

Quick Overview for PRC1 antibody (AA 1-233) (ABIN3043445)

Target: PRC1 (Protein Regulator of Cytokinesis 1 (PRC1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRC1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PRC1 Antibody

Binding Specificity: AA 1-233

Purpose: Anti-PRC1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PRC1 Antibody Picoband® (ABIN3043445). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PRC1 recombinant protein (Position: M1-E233). Human PRC1 shares 85% amino acid (aa) sequence identity with mouse PRC1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. "Entrez Gene: PRC1 protein regulator of cytokinesis 1". 2. Hartz, P. A. Personal Communication. Baltimore, Md. 2/1/2005. 3. Jiang W, Jimenez G, Wells NJ, Hope TJ, Wahl GM, Hunter T, Fukunaga R (1998). "PRC1: a human mitotic spindle-associated CDK substrate protein required for cytokinesis.". Mol. Cell. 2 (6): 877-85.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PRC1

Target: PRC1 (Protein Regulator of Cytokinesis 1 (PRC1))

Alternative Name: PRC1

Background:

Synonyms: Protein regulator of cytokinesis 1 ,PRC1 ,

Tissue Specificity: Overexpressed in bladder cancer cells (PubMed:17409436). .

Background: Protein Regulator of cytokinesis 1 (PRC1) is a protein that in humans is encoded by the PRC1 gene and is involved in cytokinesis. This gene is mapped to chromosome 15q26.1 based on an alignment of the PRC1 sequence with the genomic sequence. PRC1 protein is expressed at relatively high levels during S and G2/M phases of the cell cycle before dropping dramatically after mitotic exit and entrance into G1 phase. It is located in the nucleus during interphase, becomes associated with the mitotic spindle in a highly dynamic manner during anaphase, and localizes to the cell midbody during cytokinesis. And this protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). It is necessary for polarizing parallel microtubules and concentrating the factors responsible for contractile ring assembly. Alternative splicing results in multiple transcript variants.

Molecular Weight: 75 kDa, 80 kDa

Gene ID: 9055

UniProt: O43663