NMI antibody (AA 2-307)

Catalog No. ABIN3043451

The Rabbit Polyclonal anti-NMI antibody has been validated for WB, IHC, IF, FACS and ICC. It is suitable to detect NMI in samples from Human.

Quick Overview for NMI antibody (AA 2-307) (ABIN3043451)

Target: NMI (N-Myc (And STAT) Interactor (NMI))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NMI antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-NMI Antibody

Binding Specificity: AA 2-307

Purpose: Anti-N myc interactor/NMI Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-N myc interactor/NMI Antibody Picoband® (ABIN3043451). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NMI recombinant protein (Position: E2-E307). Human NMI shares 64% amino acid (aa) sequence identity with mouse NMI.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Albihn A, et al. Camptothecin-induced apoptosis is enhanced by Myc and involves PKCdelta signaling. Int J Cancer, 2007 Oct 15. 2. Fillmore RA, et al. Nmi (N-Myc interactor) inhibits Wnt/beta-catenin signaling and retards tumor growth. Int J Cancer, 2009 Aug 1. 3. Lebrun SJ, et al. Interferon-induced upregulation and cytoplasmic localization of Myc-interacting protein Nmi. J Interferon Cytokine Res, 1998 Sep.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for NMI

Target: NMI (N-Myc (And STAT) Interactor (NMI))

Alternative Name: NMI

Background:

Synonyms: N-myc-interactor,Nmi,N-myc and STAT interactor,NMI,

Tissue Specificity: Expressed in all adult and fetal tissues except brain and skin. More abundant in fetal tissues especially liver.

Background: NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias.

Molecular Weight: 35 kDa

Gene ID: 9111

UniProt: Q13287