Tumor Protein p73 antibody (AA 1-198)

Catalog No. ABIN3043469

The Rabbit Polyclonal anti-Tumor Protein p73 antibody has been validated for WB and IHC. It is suitable to detect Tumor Protein p73 in samples from Human, Mouse and Rat. There are 2+ publications available.

Quick Overview for Tumor Protein p73 antibody (AA 1-198) (ABIN3043469)

Target: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tumor Protein p73 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Tumor Protein p73 Antibody

Binding Specificity: AA 1-198

Purpose: Anti-p73/TP73 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-p73/TP73 Antibody Picoband® (ABIN3043469). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human p73 recombinant protein (Position: M1-E198). Human p73 shares 96% amino acid (aa) sequence identity with mouse p73.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human
1. Kaghad, M., Bonnet, H., Yang, A., Creancier, L., Biscan, J.-C., Valent, A., Minty, A., Chalon, P., Lelias, J.-M., Dumont, X., Ferrara, P., McKeon, F., Caput, D. : Monoallelically expressed gene related to p53 at 1p36, a region frequently deleted in neuroblastoma and other human cancers. Cell 90: 809-819, 1997. 2. Agami, R., Blandino, G., Oren, M., Shaul, Y. : Interaction of c-Abl and p73-alpha and their collaboration to induce apoptosis. Nature 399: 809-813, 1999. 3. Yuan, Z.-M., Shioya, H., Ishiko, T., Sun, X., Gu, J., Huang, Y. Y., Lu, H., Kharbanda, S., Weichselbaum, R., Kufe, D. : p73 is regulated by tyrosine kinase c-Abl in the apoptotic response to DNA damage. Nature 399: 814-817, 1999.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Tumor Protein p73 antibody (ABIN3043469)

Guo, Nan, Hu, Meng, Hui, Zhang, Qin, Sui: "Prognostic significance of co-expression of nm23 and p57 protein in hepatocellular carcinoma." in: Hepatology research : the official journal of the Japan Society of Hepatology, Vol. 40, Issue 11, pp. 1107-16, (2010) (PubMed).

Qin, Nan, Yang, Jing, Ruan, Li, Xu, Guo, Sui, Wei: "Expression and clinical significance of TAp73alpha, p53, PCNA and apoptosis in hepatocellular carcinoma." in: World journal of gastroenterology, Vol. 11, Issue 18, pp. 2709-13, (2005) (PubMed).

Target Details for Tumor Protein p73

Target: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Alternative Name: TP73

Background:

Synonyms: Tumor protein p73,p53-like transcription factor,p53-related protein,TP73,P73,

Tissue Specificity: Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue. .

Background: p73, also known as tumor protein 73 (TP73), encodes a member of the p53 family of transcription factors involved in cellular responses to stress and development. It is mapped to a region on chromosome 1p36 that is frequently deleted in neuroblastoma and other tumors, and thought to contain multiple tumor suppressor genes. The demonstration that this gene is monoallelically expressed (likely from the maternal allele), supports the notion that it is a candidate gene for neuroblastoma. Furthermore, recent finding are suggesting that over-expression of transcription factors involved in cell cycle regulation and synthesis of DNA in mammalian cells (e.g.: E2F-1) induces the expression of p73. In addition, p73 is a substrate of the c-Abl kinase and that the ability of c-Abl to phosphorylate p73 is markedly increased by gamma-irradiation.

Sequence Similarities: Belongs to the p53 family.

Molecular Weight: 73 kDa

Gene ID: 7161

UniProt: O15350

Pathways: Regulation of Cell Size, Positive Regulation of Response to DNA Damage Stimulus