CCL7 antibody (AA 24-97)

Catalog No. ABIN3043479

This anti-CCL7 antibody is a Rabbit Polyclonal antibody detecting CCL7 in WB and ELISA. Suitable for Mouse.

Quick Overview for CCL7 antibody (AA 24-97) (ABIN3043479)

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CCL7 Antibody

Binding Specificity: AA 24-97

Purpose: Anti-MCP3/CCL7 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-MCP3/CCL7 Antibody Picoband® (ABIN3043479). Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse MCP3 recombinant protein (Position: Q24-P97). Mouse MCP3 shares 60% and 95% amino acid (aa) sequences identity with human and rat MCP3, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, -
ELISA, 0.1-0.5 μg/mL, -
1. Ong, V. H., Evans, L. A., Shiwen, X., Fisher, I. B., Rajkumar, V., Abraham, D. J., Black, C. M., Denton, C. P.Monocyte chemoattractant protein 3 as a mediator of fibrosis: overexpression in systemic sclerosis and the type 1 tight-skin mouse.Arthritis Rheum. 48: 1979-1991, 2003. 2. McQuibban, G. A., Gong, J.-H., Tam, E. M., McCulloch, C. A. G., Clark-Lewis, I., Overall, C. M.Inflammation dampened by gelatinase A cleavage of monocyte chemoattractant protein-3.Science 289: 1202-1206, 2000.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CCL7

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Alternative Name: CCL7

Background:

Synonyms: C-C motif chemokine 7,Intercrine/chemokine MARC,Monocyte chemoattractant protein 3,Monocyte chemotactic protein 3,MCP-3,Protein FIC,Small-inducible cytokine A7,Ccl7,Fic, Mcp3, Scya7,

Tissue Specificity: Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Expressed in a variety of tissues. Overexpressed in tumors, including melanomas, cancers of the colon, pancreas prostate and kidney. .

Background: Chemokine (C-C motif) ligand 7 (CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3 (MCP3). It belongs to the C-C chemokine family. By fluorescence in situ hybridization, MCP3 gene is mapped to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, it has been found that matrix metalloproteinases are both effectors and regulators of the inflammatory response.

Sequence Similarities: Belongs to the intercrine beta (chemokine CC) family.

Molecular Weight: 36 kDa

Gene ID: 20306

UniProt: Q03366