Cyclin A1 antibody (AA 64-263)

Catalog No. ABIN3043480

Product Details anti-Cyclin A1 Antibody

Target: Cyclin A1 (CCNA1)

Binding Specificity: AA 64-263

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purpose: Anti-Cyclin A1/CCNA1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Cyclin A1/CCNA1 Antibody Picoband® (ABIN3043480). Tested in IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Cyclin A1 recombinant protein (Position: D64-E263). Human Cyclin A1 shares 78.5% and 79.5% amino acid (aa) sequence identity with mouse and rat Cyclin A1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
1. "Entrez Gene: CCNA1 cyclin A1". 2. Yang R, Morosetti R, Koeffler HP (March 1997). "Characterization of a second human cyclin A that is highly expressed in testis and in several leukemic cell lines".Cancer Res. 57 (5): 913-20.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Cyclin A1 antibody (ABIN3043480)

Zeng, Ma, Chen, Qiu, Wu, Ling, Zeng: "Autocrine fibronectin from differentiating mesenchymal stem cells induces the neurite elongation in vitro and promotes nerve fiber regeneration in transected spinal cord injury." in: Journal of biomedical materials research. Part A, Vol. 104, Issue 8, pp. 1902-11, (2017) (PubMed).

Yuan, Zheng, Li, Xiang, Gao, Ran, Sun, Huang, Xie, Du, Xiao: "Overexpression of miR-30a in lung adenocarcinoma A549 cell line inhibits migration and invasion via targeting EYA2." in: Acta biochimica et biophysica Sinica, Vol. 48, Issue 3, pp. 220-8, (2016) (PubMed).

Target Details for Cyclin A1

Target: Cyclin A1 (CCNA1)

Alternative Name: CCNA1 (CCNA1 Products)

Background:

Synonyms: Cyclin-A1,CCNA1,

Tissue Specificity: Very high levels in testis and very low levels in brain. Also found in myeloid leukemia cell lines.

Background: Cyclin-A1 is a protein that in humans is encoded by the CCNA1 gene. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin encoded by this gene was shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. Also this cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. Multiple transcript variants encoding different isoforms have been found for this gene.

Sequence Similarities: Belongs to the cyclin family. Cyclin AB subfamily.

Molecular Weight: 55 kDa

Gene ID: 8900

UniProt: P78396

Pathways: Apoptosis, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA