CD8 alpha antibody (AA 22-187)

Catalog No. ABIN3043499

Product Details anti-CD8 alpha Antibody

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Binding Specificity: AA 22-187

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD8 alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for T-cell surface glycoprotein CD8 alpha chain(CD8A) detection. Tested with WB, IHC-P in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for T-cell surface glycoprotein CD8 alpha chain(CD8A) detection. Tested with WB, IHC-P in Human.
Gene Name: CD8a molecule
Protein Name: T-cell surface glycoprotein CD8 alpha chain

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD8 alpha recombinant protein (Position: S22-A187). Human CD8 alpha shares 49% and 47% amino acid (aa) sequence identity with mouse and rat CD8 alpha, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for CD8 alpha is approximately 0.2 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-CD8 alpha antibody (ABIN3043499)

Xu, Chen, Zhao, Huang, Duan, Tong, Zhang, Li, Chang, Chen: "The CD8? gene in duck (Anatidae): cloning, characterization, and expression during viral infection." in: Molecular biology reports, Vol. 42, Issue 2, pp. 431-9, (2015) (PubMed).

Xu, Tan, Wang, Wang, Li, Wu, Chen, Wu, Tan: "Elevated survivin expression in peripheral blood mononuclear cells is central to collateral formation in coronary chronic total occlusion." in: International journal of molecular medicine, Vol. 35, Issue 6, pp. 1501-10, (2015) (PubMed).

Zhang, Chen, Lao, Huang: "Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients." in: World journal of gastroenterology, Vol. 10, Issue 10, pp. 1436-9, (2005) (PubMed).

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8A (CD8A Products)

Synonyms: cd8 antibody, LOC100136450 antibody, BB154331 antibody, Ly-2 antibody, Ly-35 antibody, Ly-B antibody, Lyt-2 antibody, zgc:136643 antibody, CD8 antibody, Leu2 antibody, MAL antibody, p32 antibody, RHACD8-4 antibody, CD8a molecule antibody, uncharacterized LOC100125537 antibody, CD8 alpha antibody, CD8 antigen, alpha chain antibody, T-cell surface glycoprotein CD8 alpha chain antibody, Cd8a antibody, LOC100125537 antibody, LOC100136450 antibody, CD8A antibody, cd8a antibody, LOC100126556 antibody

Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene also encodes the CD8 alpha chain isoforms.

Synonyms: alpha polypeptide (p32) antibody|CD 8a antibody|CD8 alpha chain antibody|CD8 antibody|CD8 antigen alpha chain antibody|CD8 antigen alpha polypeptide antibody|CD8 antigen alpha polypeptide (p32) antibody|CD8a antibody|CD8a antigen antibody|CD8a molecule antibody|CD8A_HUMAN antibody|Leu 2 antibody|Leu2 antibody|Leu2 T lymphocyte antigen antibody|Ly3 antibody|LYT3 antibody|MAL antibody|OKT8 T cell antigen antibody|OTTHUMP00000160760 antibody|OTTHUMP00000160764 antibody|OTTHUMP00000203528 antibody|OTTHUMP00000203721 antibody|p32 antibody|T cell antigen Leu2 antibody|T cell co receptor antibody|T cell coreceptor antibody|T cell surface glycoprotein CD8 alpha chain antibody|T lymphocyte differentiation antigen T8/Leu 2 antibody|T lymphocyte differentiation antigen T8/Leu2 antibody|T-cell surface glycoprotein CD8 alpha chain antibody|T-lymphocyte differentiation antigen T8/Leu-2 antibody|T8 T cell antigen antibody|T8/Leu-2 T-lymphocyte differentiation antigen antibody

Gene ID: 925

UniProt: P01732

Pathways: TCR Signaling