PKC beta antibody (AA 542-671)

Catalog No. ABIN3043550

Product Details anti-PKC beta Antibody

Target: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Binding Specificity: AA 542-671

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC beta antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Protein kinase C beta type(PRKCB) detection. Tested with WB in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Protein kinase C beta type(PRKCB) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: protein kinase C, beta
Protein Name: Protein kinase C beta type

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PKC beta 1 recombinant protein (Position: E542-V671). Human PKC beta 1 shares 100% amino acid (aa) sequence identity with both mouse and rat PKC beta 1.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for PKC beta 1 is approximately 0.1 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-PKC beta antibody (ABIN3043550)

Gan, Wang, Zhao, Wu, Yang, Peng: "5-(Bis(3-(2-hydroxyethyl)-1H-indol-2-yl)methyl)-2-hydroxybenzoic acid (BHIMHA): showing a strategy of designing drug to block lung metastasis of tumors." in: Drug design, development and therapy, Vol. 10, pp. 711-21, (2016) (PubMed).

Wang, Qin, Deng, Yao: "Different localization and expression of protein kinase C-beta in kidney cortex of diabetic nephropathy mice and its role in telmisartan treatment." in: American journal of translational research, Vol. 7, Issue 6, pp. 1116-25, (2015) (PubMed).

Target Details for PKC beta

Target: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Alternative Name: PRKCB (PRKCB Products)

Synonyms: PKC-beta antibody, PKCB antibody, PRKCB1 antibody, PRKCB2 antibody, A130082F03Rik antibody, PKC-Beta antibody, Pkcb antibody, Prkcb1 antibody, Prkcb2 antibody, PKC-B antibody, Prkcb antibody, prkcb1 antibody, zgc:63591 antibody, PKC antibody, PRKCB_tv2 antibody, Pkc53E antibody, prkcb1l antibody, zgc:64063 antibody, protein kinase C beta antibody, protein kinase C, beta antibody, protein kinase C, beta b antibody, protein kinase C, beta a antibody, PRKCB antibody, Prkcb antibody, prkcbb antibody, prkcba antibody

Background: Protein kinase C beta type is an enzyme that in humans is encoded by the PRKCB gene. It is a member of the protein kinase C (PKC) gene family. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. It has been found that PRKCB activated by oxidative conditions in the cell, induces phosphorylation of p66(SHC) and triggers mitochondrial accumulation of the protein after it is recognized by the prolyl isomerase PIN1.

Synonyms: KPCB_HUMAN antibody|MGC41878 antibody|PKC B antibody|PKC beta antibody|PKC-B antibody|PKC-beta antibody|PKCB antibody|PRKC B antibody|PRKC B1 antibody|PRKCB antibody|PRKCB I antibody|PRKCB1 antibody|PRKCB2 antibody|Protein kinase C beta 1 antibody|Protein kinase C beta antibody|Protein kinase C beta type antibody|protein kinase C, beta 1 polypeptide antibody|protein kinase C, beta-1 antibody

Gene ID: 5579

UniProt: P05771

Pathways: WNT Signaling, TCR Signaling, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded Protein Response, BCR Signaling