PBK antibody (AA 71-322)

Catalog No. ABIN3043592

The Rabbit Polyclonal anti-PBK antibody has been validated for WB, IHC, IF, FACS and ICC. It is suitable to detect PBK in samples from Human, Mouse and Rat.

Quick Overview for PBK antibody (AA 71-322) (ABIN3043592)

Target: PBK (PDZ Binding Kinase (PBK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PBK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-PBK Antibody

Binding Specificity: AA 71-322

Purpose: Anti-PBK Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-PBK Antibody Picoband® (ABIN3043592). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PBK recombinant protein (Position: N71-V322). Human PBK shares 89% amino acid (aa) sequence identity with mouse PBK.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Abe Y, Matsumoto S, Kito K, Ueda N (Aug 2000). "Cloning and expression of a novel MAPKK-like protein kinase, lymphokine-activated killer T-cell-originated protein kinase, specifically expressed in the testis and activated lymphoid cells". J Biol Chem 275 (28): 21525-31. 2. "Entrez Gene: PBK PDZ binding kinase" 3. Park, J.-H., Lin, M.-L., Nishidate, T., Nakamura, Y., Katagiri, T. PDZ-binding kinase/T-LAK cell-originated protein kinase, a putative cancer/testis antigen with an oncogenic activity in breast cancer. Cancer Res. 66: 9186-9195, 2006.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PBK

Target: PBK (PDZ Binding Kinase (PBK))

Alternative Name: PBK

Background:

Synonyms: Lymphokine-activated killer T-cell-originated protein kinase,2.7.12.2,Cancer/testis antigen 84,CT84,MAPKK-like protein kinase,Nori-3,PDZ-binding kinase,Spermatogenesis-related protein kinase,SPK,T-LAK cell-originated protein kinase,PBK,TOPK,

Tissue Specificity: Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules. .

Background: Lymphokine-activated killer T-cell-originated protein kinase, also known as TOPK or PBK, is an enzyme that in humans is encoded by the PBK gene. The protein encoded by this gene is a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. It is mapped to 8p21.1. PBK can mediate cell growth through histone H3 modification. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis.

Sequence Similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.

Molecular Weight: 40 kDa

Gene ID: 55872