MUC4 antibody (AA 1249-1444)

Catalog No. ABIN3043595

The Rabbit Polyclonal anti-MUC4 antibody has been validated for WB. It is suitable to detect MUC4 in samples from Human and Rat.

Quick Overview for MUC4 antibody (AA 1249-1444) (ABIN3043595)

Target: MUC4 (Mucin 4, Cell Surface Associated (MUC4))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MUC4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MUC4 Antibody

Binding Specificity: AA 1249-1444

Purpose: Anti-MUC4 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-MUC4 Antibody Picoband® (ABIN3043595). Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MUC4 recombinant protein (Position: M1249-D1444). Human MUC4 shares 69% and 36% amino acid (aa) sequences identity with mouse and rat MUC4, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Rat, Human
1. Porchet N, Nguyen VC, Dufosse J, Audie JP, Guyonnet-Duperat V, Gross MS, Denis C, Degand P, Bernheim A, Aubert JP (May 1991). "Molecular cloning and chromosomal localization of a novel human tracheo-bronchial mucin cDNA containing tandemly repeated sequences of 48 base pairs". Biochem Biophys Res Commun 175 (2): 414-22. 2. Chang CY, Chang HW, Chen CM, Lin CY, Chen CP, Lai CH, Lin WY, Liu HP, Sheu JJ, Tsai FJ (2011). "MUC4 gene polymorphisms associate with endometriosis development and endometriosis-related infertility". BMC Med 9: 19. 3. Carraway KL, Perez A, Idris N, et al. (2003). "MUC4/sialomucin complex, the intramembrane ErbB2 ligand, in cancer and epithelia: to protect and to survive.". Prog. Nucleic Acid Res. Mol. Biol. 71: 149-85.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MUC4

Target: MUC4 (Mucin 4, Cell Surface Associated (MUC4))

Alternative Name: MUC4

Background:

Synonyms: Mucin-4,MUC-4,Ascites sialoglycoprotein,ASGP,Pancreatic adenocarcinoma mucin,Testis mucin,Tracheobronchial mucin,Mucin-4 alpha chain,Ascites sialoglycoprotein 1,ASGP-1,Mucin-4 beta chain,Ascites sialoglycoprotein 2,ASGP-2,MUC4,

Tissue Specificity: Expressed in the thymus, thyroid, lung, trachea, esophagus, stomach, small intestine, colon, testis, prostate, ovary, uterus, placenta, and mammary and salivary glands. Expressed in carcinomas arising from some of these epithelia, such as lung cancers, squamous cell carcinomas of the upper aerodigestive tract, mammary carcinomas, biliary tract, colon, and cervix cancers. Minimally or not expressed in the normal pancreas or chronic pancreatitis, but is highly expressed in pancreatic tumors and pancreatic tumor cell lines. .

Background: Mucin 4, also known as MUC4, is a mucin protein that in humans is encoded by the MUC4 gene. Like other mucins, Mucin 4 is a high-molecular weight glycoprotein. It is mapped to 3q29. In normal functioning, Mucin 4 is known to play anti-adhesive roles in the body, such as in lubricating the reproductive lining. It is found in the respiratory tract, particularly in the trachea and lung and the digestive tract, in the esophagus and colon, as well as in the visual and auditory systems. Mucin 4 serves to protect and lubricate the epithelium, which facilitates transport and traps foreign particles. It is found to be overexpressed during the luteal phase of menstruation. Mucin 4 may also have a role in fetal morphogenic development.

Sequence Similarities: Contains 1 AMOP domain.

Molecular Weight: 234 kDa

Gene ID: 4585

UniProt: Q99102

Pathways: Regulation of Leukocyte Mediated Immunity