PER2 antibody (AA 13-330)

Catalog No. ABIN3043602

This anti-PER2 antibody is a Rabbit Polyclonal antibody detecting PER2 in WB. Suitable for Human.

Quick Overview for PER2 antibody (AA 13-330) (ABIN3043602)

Target: PER2 (Period Circadian Protein 2 (PER2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PER2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PER2 Antibody

Binding Specificity: AA 13-330

Purpose: Anti-PER2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-PER2 Antibody Picoband® (ABIN3043602). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PER2 recombinant protein (Position: N13-K330). Human PER2 shares 84% and 83% amino acid (aa) sequence identity with mouse and rat PER2, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Chen, R., Schirmer, A., Lee, Y., Lee, H., Kumar, V., Yoo, S.-H., Takahashi, J. S., Lee, C. Rhythmic PER abundance defines a critical nodal point for negative feedback within the circadian clock mechanism. Molec. Cell 36: 417-430, 2009. 2. Fu, L., Pelicano, H., Liu, J., Huang, P., Lee, C. C. The circadian gene Period2 plays an important role in tumor suppression and DNA damage response in vivo. Cell 111: 41-50, 2002. Note: Erratum: 111: 1055 only, 2002. 3. Gery, S., Virk, R. K., Chumakov, K., Yu, A., Koeffler, H. P. The clock gene Per2 links the circadian system to the estrogen receptor. Oncogene 26: 7916-7920, 2007.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PER2

Target: PER2 (Period Circadian Protein 2 (PER2))

Alternative Name: PER2

Background:

Synonyms: Period circadian protein homolog 2,hPER2,Circadian clock protein PERIOD 2,PER2,KIAA0347,

Tissue Specificity: Widely expressed. Found in heart, brain, placenta, lung, liver, skeleatal muscle, kidney and pancreas. High levels in skeletal muscle and pancreas. Low levels in lung. Isoform 2 is expressed in keratinocytes (at protein level). .

Background: The PER2 gene encodes the period circadian protein homolog 2 protein in humans. This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. PER2 is mapped to 2q37.3. It has been showed that PER2 can positively stimulate activity of the Bmal1-Npas2 transcription complex and, in turn, Npas2 transcriptionally regulates delta-aminolevulinate synthase. PER2 is involved in a negative-feedback loop that attenuates estrogen stimulation and links the circadian cycle to estrogen receptor signaling. The rhythmic levels of PER2 are critical for circadian oscillations in cells and intact organisms. What's more, the PER2 gene also functions in tumor suppression by regulating DNA damage-responsive pathways.

Sequence Similarities: Contains 1 PAC (PAS-associated C-terminal) domain.

Molecular Weight: 130 kDa

Gene ID: 8864

UniProt: O15055